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• 20.109(F08): Mod 2 Day 5 Probe western, isolate RNA
  ...tual manipulations of the Western are not time consuming, we'll also begin work on another portion of this experimental module, namely the microarray analy ...ou should photograph or scan your plates to include when you write up this work as a research article.
  20 KB (3,266 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray
  ...polymerases, this enzyme can only add sequence to an existing chain and so needs a short “primer” to begin synthesis. To perform the cDNA synthesis, you ...NA only” pipet tips and solutions and wiping down your bench. You should work on a fresh piece of benchpaper and remember to wear gloves when working wit
  17 KB (2,864 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 2 Day 8 Microarray data analysis
  ...edit and complete the information associated with your terrific microarray work!
  7 KB (1,156 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 3 Day 4 Phage by design
  The work for today will very much depend on the outcome of the meeting with the Graf * needed resources to complete the work (1 slide)
  10 KB (1,689 words) - 19:06, 28 July 2015
• 20.109(F08):DNA engineering lab report guidelines
  ...figures, but if you do so, you must include the reference to the published work. Figures you use should not be downloaded from a web site. ... be used to detect homologous recombination in mammalian cells. The reader needs to know what the goal is: which plasmid did you set out to create? Part B o
  9 KB (1,494 words) - 19:06, 28 July 2015
• 20.109(F08):Lab basics
  *Procedures for doing tissue culture work.
  883 B (115 words) - 19:06, 28 July 2015
• 20.109(F08):Guidelines for writing up your research
  ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.
  12 KB (1,922 words) - 19:06, 28 July 2015
• 20.109(F08):DNA engineering powerpoint pitch guidelines
  ...r this assignment, though you will receive feedback on the quality of your work.
  2 KB (319 words) - 19:06, 28 July 2015
• 20.109(F08): ECD experimental variations
  ...ments that haven't been done before and for letting everyone know how they work.
  1 KB (179 words) - 19:06, 28 July 2015
• 20.109(F08):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  5 KB (761 words) - 19:06, 28 July 2015
• 20.109(S09): Transfection (Day5)
  ... well-deserved break, I hope you're ready to head full force back into lab work and analysis. Today you will revisit MES cell culture, transfecting these c
  17 KB (2,783 words) - 19:34, 28 July 2015
• 20.109(S09): Luciferase assays and RNA prep (Day6)
  ...makes it science. There may be other ideas and explanations for how things work but when the preponderance of evidence supports an idea, it becomes the sci Scientific work generates evidence-based, internally consistent, and well-tested explanatio
  14 KB (2,345 words) - 19:34, 28 July 2015
• 20.109(S09):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  5 KB (788 words) - 19:34, 28 July 2015
• 20.109(S09):Guidelines for writing up your research
  ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.
  13 KB (2,033 words) - 19:34, 28 July 2015
• 20.109(F09): Mod 2 Day 3 Tools for system engineering
  ...en defined through traditional scientific studies of EnvZ, for example the work from Tom Silhavy's lab( [http://openwetware.org/wiki/PMID:_9721293 ] and [[ ...ld examine the bacterial photograph you set up last time and document your work and your ideas about the experiment.
  24 KB (3,897 words) - 19:39, 28 July 2015
• 20.109(F09): Mod 2 Day 5 Assessing re-tuned system
  ...in, YPYDVPDYA, in sufficient for antibody recognition--an experiment we'll work on today. ...el should take approximately one hour to run. During that hour, you should work on part two of today's protocol.<br>
  17 KB (2,716 words) - 19:39, 28 July 2015
• 20.109(F09): Mod 3 Day 2 Phage nanowires
  ... gold and silver. You will have time while these reactions are going on to work on the research proposal idea you've got started with your lab partner. * needed resources to complete the work
  7 KB (1,146 words) - 19:39, 28 July 2015
• 20.109(S09)
  ...ts at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of
  3 KB (412 words) - 19:33, 28 July 2015
• 20.109(S09):Module 1
  ...iscussions during the development of this module, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module
  3 KB (497 words) - 19:33, 28 July 2015
• 20.109(S09):Protein-level analysis (Day6)
  ...lets return to thinking about the structure of cartilage for a bit. Our work in this module has focused on chondrocytes themselves (viability and morpho ... diluted antibody per well. Incubate for 90 min (at room temperature), and work on Parts 2 and 3 of today's protocol.
  10 KB (1,561 words) - 19:33, 28 July 2015