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• 20.109(S08):Lab basics
  *Procedures for doing tissue culture work.
  903 B (119 words) - 14:24, 5 June 2015
• 20.109(S08):Student presentations (M2D8)
  ...f your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Spe
  9 KB (1,472 words) - 14:24, 5 June 2015
• 20.109(S08):Lab tour
  Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa
  22 KB (3,631 words) - 14:24, 5 June 2015
• 20.109(S08):Induce protein expression (Day4)
  As evidenced by Nagais work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will look at the sequencing results fo
  15 KB (2,475 words) - 14:25, 5 June 2015
• 20.109(S08):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  5 KB (761 words) - 14:25, 5 June 2015
• 20.109(S08):Guidelines for writing a lab report
  ... list the name of your partner on your report since she contributed to the work. ... trends, raw data (like a picture of a gel), or a model that explains your work.
  12 KB (1,912 words) - 14:25, 5 June 2015
• 20.109(S08):Guidelines for working in the tissue culture facility
  ...ut most are common practice and will be good habits for any tissue culture work you do. ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary.
  7 KB (1,095 words) - 14:25, 5 June 2015
• 20.109(S08):Data analysis (Day7)
  ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), then move on to your mutant ...SingleKD]], and [[Media:Fit_KDn.m| Fit_KDn]]. Move them to the C:/MATLAB72/work folder on your PC.
  14 KB (2,336 words) - 14:25, 5 June 2015
• 20.109(S07): Yeast transformation
  ...lt projects should be tackled with the idea that they can, should and will work. Attitude alone, however, does not overcome pitfalls and there is much to b ...distinguish these possible explanations but it can tell you that something needs to be re-worked.
  19 KB (3,251 words) - 15:32, 15 June 2015
• 20.109(S07):General lab policy
  ... your partner and to others in the lab if you are not up to speed when the work begins. ... have cut yourself (even if you consider it minor), if something broke and needs cleaning up, or if you are on fire.
  2 KB (380 words) - 15:32, 15 June 2015
• 20.109(S07):Lab tour
  Our lab is beautifully equipped. We have a fume hood for work that generates hazardous vapors. We have a back room where dishes can be wa
  22 KB (3,724 words) - 15:32, 15 June 2015
• 20.109(S07):Assignments
  ...ake a figure using the data you have collected. <font color = red> You can work with your lab partner and friends on these assignments but you will hand in <font color= red><b>Late work will be penalized 1/3 of a letter grade for each day late and will not be a
  2 KB (279 words) - 15:32, 15 June 2015
• 20.109(S07): Start-up expression engineering
  ...gulating protein stability, localization and modifications. Thus, from the work we've done so far this term, you may have the idea that gene expression is ...tocol for designing the reverse primer is just one method of many that can work. For example, taking the reverse complement can be done at any stage, makin
  28 KB (4,311 words) - 15:32, 15 June 2015
• 20.109(S07): Bio-material engineering/Rescreening gold binders
  #* needed resources to complete the work (1 slide) ... vary depending on the particulars of your proposal. You will have time to work on the presentation in lab next time as well as ask questions about the for
  5 KB (887 words) - 15:32, 15 June 2015
• 20.109(S07): Expression engineering report
  You are asked to write a formal lab report detailing your work in this module. Specifics for each section of this report are detailed belo *conclusions you can draw from your work, including any uncertainties
  3 KB (479 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 3
  When you have a break from the work described below, be sure to examine the plaques you plated last time. Recor ...assay, what is the titer of each stock solution of phage? Please show your work. If the plaques appeared different, please consider how the phage genomes d
  12 KB (2,079 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for editing OpenWetWare
  ==Sign your work==
  3 KB (497 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  4 KB (732 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 5
  ...el should take approximately one hour to run. During that hour, you should work on parts two and three of today's protocol.<br>
  11 KB (1,756 words) - 15:32, 15 June 2015
• 20.109(S07):Guidelines for working in the tissue culture facility
  ...y with 70% ethanol. Start from the back and proceed forward. Swab during work if necessary. ...n the hood and maintain plenty of clear space in the center of the hood to work in.
  6 KB (1,050 words) - 15:32, 15 June 2015