Optical Microscopy: Part 3 Report Outline
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Revision as of 19:22, 17 September 2014 by MAXINE JONAS (Talk | contribs)
- Disrupting and imaging the actin network
- Procedure
- Document sample preparation, including difficulties, and recording conditions (particularly exposure time and gain selected in the camera settings)
- Data
- Include images of mouse embryonic fibroblasts + and - CytoD.
- Analysis and Results
- What contrast do you unveil between the + and - CytoD conditions?
- Discussion
- Can you quantify the morphological and physiological effect of CytoD treatment? What parameters would you choose to measure and report?
- Procedure
- Resolution
- Procedure
- Document the samples you used and how you captured images (camera settings, software used, etc…)
- Data
- Include an image of the PSF sample with the beads used for resolution measurement indicated.
- Analysis and Results
- Report the resolution you measured. Make sure to include N and a measure of uncertainty.
- Show sample Gaussian fits.
- Explain the Matlab algorithm used for data analysis.
- Discussion
- Compare the measured value to the theoretical value.
- Include a thorough discussion of error sources.
- Procedure
- Stability
- Procedure
- Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)
- Data
- Show an example frame from the stability movie.
- Provide X-Y plots of difference tracks for pairs of fixed particles.
- Plot MSD versus time interval τ for sum and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.
- Analysis and Results
- Provide a bullet point outline of data analysis methodology.
- Include a thorough discussion of error sources.
- Discussion
- What are the benefits and drawbacks of differential tracking?
- Procedure