Difference between revisions of "Spring 11:QRT-PCR"
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Kristin Kuhn (Talk | contribs) (→Weekly goals) |
Kristin Kuhn (Talk | contribs) |
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== To-Do == | == To-Do == | ||
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* research what to order for heated lid | * research what to order for heated lid | ||
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* 4/22: Setup is ready for QRT-PCR. (i.e., the mechanical setup works) | * 4/22: Setup is ready for QRT-PCR. (i.e., the mechanical setup works) | ||
* 4/29: Successful QRT-PCR from the side. | * 4/29: Successful QRT-PCR from the side. | ||
| − | * 5/6: the top. Now which one was better? | + | * 5/6: QRT-PCR from the top. Now which one was better? |
== Progress Notes == | == Progress Notes == | ||
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| + | ==== 3/20/11 ==== | ||
| + | Based on model system (blue LED + ND filter), I don't think gain is high enough. Increased gain of transimpedance amp from 2.5e4 to 2.5e5. | ||
==== 3/18/11 ==== | ==== 3/18/11 ==== | ||
Revision as of 23:28, 20 March 2011
Looking for weekly reports?
To-Do
- research what to order for heated lid
Weekly goals
- 3/25: Optics finished. Code started.
- 4/1: Heated lid finished.
- 4/8: Updated heat transfer function. Successful PCR.
- 4/15: QRT-PCR code finished.
- 4/22: Setup is ready for QRT-PCR. (i.e., the mechanical setup works)
- 4/29: Successful QRT-PCR from the side.
- 5/6: QRT-PCR from the top. Now which one was better?
Progress Notes
3/20/11
Based on model system (blue LED + ND filter), I don't think gain is high enough. Increased gain of transimpedance amp from 2.5e4 to 2.5e5.
3/18/11
Optical setup appears to be working.
3/16/11
Added dichroic mirror.
Question: how can I verify the optical setup? it seems to respond to driving signal, not fluorescence.
TODO: put away 25mm lens
