Difference between revisions of "20.109(S13): TA notes for module 1"
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(New page: {{Template:20.109(S13)}} <div style="padding: 10px; width: 640px; border: 5px solid #99FF66;"> Coming soon! ==General notes== '''Scheme:''' '''Key preparation:''' ===Day 1=== '''Ma...) |
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==General notes== | ==General notes== | ||
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| + | See also GoogleDoc for aliquoting amounts. | ||
'''Scheme:''' | '''Scheme:''' | ||
'''Key preparation:''' | '''Key preparation:''' | ||
| + | |||
| + | ==Day-by-day== | ||
===Day 1=== | ===Day 1=== | ||
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'''Day of Lab (R/F):''' | '''Day of Lab (R/F):''' | ||
| + | |||
| + | '''After Lab:''' | ||
| + | |||
| + | '''How it went:''' Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most. | ||
| + | |||
| + | ===Day 2=== | ||
| + | |||
| + | '''Materials required:''' | ||
| + | *Bird samples! At 100 μL each in a '''2 mL''' tube | ||
| + | *Aliquots of components from QiaAMP stool kit (see GoogleDoc) | ||
| + | *InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one | ||
| + | |||
| + | '''Day of Lab (R/F):''' | ||
| + | *Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench | ||
| + | *Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C | ||
| + | |||
'''After Lab:''' | '''After Lab:''' | ||
'''How it went:''' | '''How it went:''' | ||
Revision as of 14:37, 10 February 2013
Coming soon!
Contents
General notes
See also GoogleDoc for aliquoting amounts.
Scheme:
Key preparation:
Day-by-day
Day 1
Materials required:
Day of Lab (R/F):
After Lab:
How it went: Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.
Day 2
Materials required:
- Bird samples! At 100 μL each in a 2 mL tube
- Aliquots of components from QiaAMP stool kit (see GoogleDoc)
- InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one
Day of Lab (R/F):
- Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
- Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
After Lab:
