Difference between revisions of "Resource list: DNA melting and PCR"
From Course Wiki
(→Real-time PCR) |
|||
| Line 24: | Line 24: | ||
*[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) (Also see: Ahsen, et. al. [http://www.clinchem.org/cgi/content/full/47/7/1331#R1 Limitations of Genotyping Based on Amplicon Melting Temperature.] Clinical Chemistry. 2001;47:1331-1332. | *[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) (Also see: Ahsen, et. al. [http://www.clinchem.org/cgi/content/full/47/7/1331#R1 Limitations of Genotyping Based on Amplicon Melting Temperature.] Clinical Chemistry. 2001;47:1331-1332. | ||
| − | == | + | ==PCR== |
| − | * Kubista, et. al. [http://www.tataa.com/files/documents/molecularaspectsofmedicine2006.pdf The real-time polymerase chain reaction] ''Molecular Aspects of Medicine'' 27 (2006) 95–125. | + | * Saiki, et. al. [http://www.sciencemag.org/cgi/reprint/239/4839/487.pdf?ijkey=06edd3fdcd16b286ed884189fcd203fd9197b18f Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase]. Science 29 January 1988: Vol. 239. no. 4839, pp. 487 - 491 DOI: 10.1126/science.2448875 |
| + | |||
| + | |||
| + | * Kubista, et. al. [http://www.tataa.com/files/documents/molecularaspectsofmedicine2006.pdf The real-time polymerase chain reaction]. ''Molecular Aspects of Medicine'' 27 (2006) 95–125. | ||
</div> | </div> | ||
Revision as of 20:27, 6 June 2010
DNA melting thermodynamics
- Ryan T. Koehler and Nicolas Peyret Thermodynamic properties of DNA sequences: characteristic values for the human genome Bioinformatics 2005 21(16):3333-3339; doi:10.1093/bioinformatics/bti530
Use of fluorescent reportes to measure DNA melting curves
- Ririe, et al. Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction. Analytical Biochemistry 245, 154–160 (1997)
- Michael T. Bjorndal and D. Kuchnir Fygenson. DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay. Biopolymers, Vol. 65, 40–44 (2002)
Applications of DNA melting curves
- Worm, et. al. In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis Clinical Chemistry 47:7 1183–1189 (2001)
- Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome. Clinical Chemistry. 2000;46:423-425.) (Also see: Ahsen, et. al. Limitations of Genotyping Based on Amplicon Melting Temperature. Clinical Chemistry. 2001;47:1331-1332.
PCR
- Saiki, et. al. Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase. Science 29 January 1988: Vol. 239. no. 4839, pp. 487 - 491 DOI: 10.1126/science.2448875
- Kubista, et. al. The real-time polymerase chain reaction. Molecular Aspects of Medicine 27 (2006) 95–125.
