Difference between revisions of "20.109(S24):M1D7"
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===Part 2: Transfer nuclear protein extractions onto membrane=== | ===Part 2: Transfer nuclear protein extractions onto membrane=== | ||
+ | ===Part 2: In-class paper discussion=== | ||
− | = | + | To further help you in preparing your Data summary, we will discuss how similar data are presented in a publication from the Koehler laboratory. |
+ | |||
+ | Chen ''et al.'' titled "[[Media:SMMAlzABPeptide Koehler2010.pdf |Small molecule microarrays enable the discovery of compounds that bind the Alzheimer's Aβ peptide]]" (2010) ''J Am Chem Soc'' 132:17015-17022. | ||
+ | |||
+ | The initial experiment presented by Chen ''et. al.'' was an SMM that identified ligands binders of the amyloid-β (Aβ) petptide. This first step is very similar to what was done to identify the hits you are testing in this module! To further assess the results of the SMM, the authors completed several follow-up experiments to test the effect of the small molecule on functionality of the Aβ petptide. | ||
+ | |||
+ | In the context of your research, this article focuses on the next step experiments that can be performed after a drug candidate is discovered from a screen. Though you can use this article as guidance as you consider the experiments that could follow your screen, remember that the specific next step experiments should be related to the protein target and drug candidate(s) identified in your project. For this exercise, the focus in on how the data are organized and presented. | ||
+ | |||
+ | '''From the Introduction''' | ||
+ | |||
+ | Consider the key components of an introduction: | ||
+ | *What is the big picture? | ||
+ | *Is the importance of this research clear? | ||
+ | *Are you provided with the information you need to understand the research? | ||
+ | *Do the authors include a preview of the key results? | ||
+ | |||
+ | '''From the Results''' | ||
+ | |||
+ | Carefully examine the figures. First, read the captions and use the information to 'interpret' the data presented within the image. Second, read the text within the results section that describes the figure. | ||
+ | *Do you agree with the conclusion(s) reached by the authors? | ||
+ | *What controls were included and are they appropriate for the experiment performed? | ||
+ | *Are you convinced that the data are accurate and/or representative? | ||
+ | |||
+ | '''From the Discussion''' | ||
+ | |||
+ | Consider the following components of a discussion: | ||
+ | *Are the results summarized? | ||
+ | *Did the authors 'tie' the data together into a cohesive and well-interpreted story? | ||
+ | *Do the authors overreach when interpreting the data? | ||
+ | *Are the data linked back to the big picture from the introduction? | ||
+ | |||
+ | <font color = #4a9152 >'''In your laboratory notebook,'''</font color> complete the following: | ||
+ | *Based on your reading and the group discussion of the article, answer the questions above. | ||
==Reagent list== | ==Reagent list== |
Revision as of 19:53, 21 February 2024
Contents
Introduction
Protocols
Part 1: Electrophorese nuclear protein extraction
Part 2: Transfer nuclear protein extractions onto membrane
Part 2: In-class paper discussion
To further help you in preparing your Data summary, we will discuss how similar data are presented in a publication from the Koehler laboratory.
Chen et al. titled "Small molecule microarrays enable the discovery of compounds that bind the Alzheimer's Aβ peptide" (2010) J Am Chem Soc 132:17015-17022.
The initial experiment presented by Chen et. al. was an SMM that identified ligands binders of the amyloid-β (Aβ) petptide. This first step is very similar to what was done to identify the hits you are testing in this module! To further assess the results of the SMM, the authors completed several follow-up experiments to test the effect of the small molecule on functionality of the Aβ petptide.
In the context of your research, this article focuses on the next step experiments that can be performed after a drug candidate is discovered from a screen. Though you can use this article as guidance as you consider the experiments that could follow your screen, remember that the specific next step experiments should be related to the protein target and drug candidate(s) identified in your project. For this exercise, the focus in on how the data are organized and presented.
From the Introduction
Consider the key components of an introduction:
- What is the big picture?
- Is the importance of this research clear?
- Are you provided with the information you need to understand the research?
- Do the authors include a preview of the key results?
From the Results
Carefully examine the figures. First, read the captions and use the information to 'interpret' the data presented within the image. Second, read the text within the results section that describes the figure.
- Do you agree with the conclusion(s) reached by the authors?
- What controls were included and are they appropriate for the experiment performed?
- Are you convinced that the data are accurate and/or representative?
From the Discussion
Consider the following components of a discussion:
- Are the results summarized?
- Did the authors 'tie' the data together into a cohesive and well-interpreted story?
- Do the authors overreach when interpreting the data?
- Are the data linked back to the big picture from the introduction?
In your laboratory notebook, complete the following:
- Based on your reading and the group discussion of the article, answer the questions above.
Reagent list
Next day: Evaluate experimental results