Difference between revisions of "Talk:20.109(F17):Module 2"
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(Created page with "===Fermentation product and gene targeted:=== ====T/R==== {| border=1px |'''Team''' |'''Ethanol (E) or Acetate (A)''' |'''Gene targeted by CRISPRi gRNA''' |'''gRNA sequence ...") |
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| Acetate | | Acetate | ||
| adhE | | adhE | ||
| − | | | + | | TTAACGCACTCGTAGAGCGTGTAAA |
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|Acetate | |Acetate | ||
|adhE | |adhE | ||
| − | | | + | |TTTACTAAAAAAGTTTAACATTATC |
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Revision as of 17:23, 30 October 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Acetate | adhE | TTAACGCACTCGTAGAGCGTGTAAA |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
| green | E | LdhA | ttgtgctataaacggcgagtttcat |
| blue | E | pta | ttcacgacaacgttcaataatcat |
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
| purple | Acetate | adhE | TTTACTAAAAAAGTTTAACATTATC |
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Ethanol | ack (indirectly, pta) | GTTTTTTTAGCCACGTATCAATTAT |
| orange | Ethanol | ldhA | ATTCAACATCACTGGAGAAAGTCTT |
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |
