Difference between revisions of "Assignment 9, Part 1: model function"
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− | Finally, put everything together into a single model function that | + | Finally, put everything together into a single model function that represents |
+ | <math>\left . V_{f,model}(t) = K_{gain} S(t) Q(t) C_{ds}(T_{sample}(t), \Delta H^\circ, \Delta S^\circ) + K_{offset}\right .</math> | ||
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+ | ''S(t)'' is the expression for photobleaching, ''Q(t)'' is the expression for thermal quenching, and ''C<sub>ds</sub>'' is the model melting curve produced by the <code>DnaFraction</code> function from part 1 of the lab. | ||
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+ | In order for the function to be compatible with MATLAB's <tt>nlinfit</tt>, the first input argument must be a vector containing all the parameters to be fitted, and the second argument must contain a vector/matrix of the independent variable(s). Don't forget to include parameters for the instrument gain and offset, and <math>\Delta H^\circ, \Delta S^\circ</math>, as well as the bleaching, quenching, and thermal time constants. | ||
<pre> | <pre> |
Revision as of 14:29, 21 August 2017
This is Part 1 of Assignment 9.
The wiki page on the DNA model function outlines a way to model the sample temperature based on the block temperature:
function SimulatedTemperatureOutput = SimulateLowPass( TimeConstant, InputData, TimeVector ) if( nargin < 3 ) TimeVector = (0:(length(InputData)-1))/10; end transferFunction = tf( 1, [TimeConstant, 1] ); initalTemperature = InputData(1); InputData = InputData - initalTemperature; SimulatedTemperatureOutput = lsim( transferFunction, InputData, TimeVector' ) + initalTemperature; end
In a similar fashion, write the following functions in MATLAB which model the phenomena of photobleaching and thermal quenching:
function SimulatedBleachingOutput = SimulatePhotobleaching( Kbleach, InputData ) ... end function SimulatedQuenchingOutput = SimulateThermalQuenching( Kquench, InputData ) ... end
Finally, put everything together into a single model function that represents $ \left . V_{f,model}(t) = K_{gain} S(t) Q(t) C_{ds}(T_{sample}(t), \Delta H^\circ, \Delta S^\circ) + K_{offset}\right . $
S(t) is the expression for photobleaching, Q(t) is the expression for thermal quenching, and Cds is the model melting curve produced by the DnaFraction
function from part 1 of the lab.
In order for the function to be compatible with MATLAB's nlinfit, the first input argument must be a vector containing all the parameters to be fitted, and the second argument must contain a vector/matrix of the independent variable(s). Don't forget to include parameters for the instrument gain and offset, and $ \Delta H^\circ, \Delta S^\circ $, as well as the bleaching, quenching, and thermal time constants.
function SimulatedOutput = Vfmodel( Parameters, InputData ) tauThermal = Parameters(1); Kbleach = Parameters(2); ... end
your code (after having tested it in part 2) |