Difference between revisions of "20.109(F07): M13.1 and MDS titers"
From Course Wiki
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*Description of M13.1 phage: | *Description of M13.1 phage: | ||
*Description of MDS strain: | *Description of MDS strain: | ||
− | *Any notes about titers presented here: | + | *Any notes about titers presented here:M13K07 control not grown in parallel |
+ | ====Plaque numbers==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS | ||
+ | |- | ||
+ | | 10-6 | ||
+ | |~1000 | ||
+ | | ND | ||
+ | |~1000 | ||
+ | |~1500 | ||
+ | |- | ||
+ | | 10-8 | ||
+ | |16 | ||
+ | |ND | ||
+ | |10 | ||
+ | |42 | ||
+ | |- | ||
+ | | 10-10 | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |} | ||
+ | </center> | ||
+ | ====Titer==== | ||
+ | <center> | ||
+ | {| border="1" | ||
+ | | | ||
+ | |phage = M13K07, strain = MG1655 | ||
+ | |phage = M13K07, strain = MDS | ||
+ | |phage = M13.1, strain = MG1655 | ||
+ | |phage = M13.1, strain = MDS | ||
+ | |- | ||
+ | | titer (PFU/ml) | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |} | ||
+ | </center> | ||
+ | |||
===Yellow Team=== | ===Yellow Team=== | ||
*Team members: | *Team members: |
Revision as of 14:44, 5 December 2007
All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]
Contents
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ||||
10-8 | ||||
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
T/R lab
Green Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Red Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Blue Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:M13K07 control not grown in parallel
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ~1000 | ND | ~1000 | ~1500 |
10-8 | 16 | ND | 10 | 42 |
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
W/F lab
Green Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Red Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Blue Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here: