Difference between revisions of "Final Project -- Nathan S Lachenmyer"
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=Confocal Microscopy= | =Confocal Microscopy= | ||
==Literature== | ==Literature== | ||
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* Handbook of biological confocal microscopy / editor, James B. Pawley. | * Handbook of biological confocal microscopy / editor, James B. Pawley. | ||
− | == | + | ==Goals and Checkpoints== |
− | + | ===Goals=== | |
− | * | + | {| border = "1" align="center" style="color: black; background-color: #ffffcc;" width="85%" class="wikitable" |
− | * | + | !Key Factors!!Proposed Technical Targets!!Requirements for Success!!Current Practice!!Associated Technical Barriers |
− | * | + | |
+ | |- align="center" | ||
+ | |style="width: 20%;"|OCLSM Cost | ||
+ | |style="width: 20%;"|< $8,000 | ||
+ | |style="width: 20%;"|< $15,000 | ||
+ | |style="width: 20%;"|> $100,000 commercial; ~$30,000 DIY | ||
+ | |style="width: 20%;"|Cheap replacement for PMT & Galvo, etc. | ||
+ | |- | ||
+ | |style="width: 20%;"|OCLSM Scan Time | ||
+ | |style="width: 20%;"|1 frame/second | ||
+ | |style="width: 20%;"|1 frame / 10 seconds | ||
+ | |style="width: 20%;"|10-50 fps | ||
+ | |style="width: 20%;"|Fast scanning mirrors + ADC time | ||
+ | |} | ||
+ | |||
+ | ===Timeline=== | ||
+ | * 2 Weeks: Brightfield Confocal Microscopy | ||
+ | * 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images | ||
+ | * 3 Weeks: Laser Scanning Features | ||
==Proposed Design== | ==Proposed Design== | ||
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==Challenges== | ==Challenges== | ||
+ | * Control software | ||
+ | * Getting good resolution | ||
− | = | + | =Weekly Updates= |
− | == | + | ==2012 April 8== |
− | * | + | * Look for PMTs and/or Galvos |
− | + | ||
− | + | ||
− | + | ||
− | == | + | ==2012 April 15== |
− | * | + | * Calculations for stuff |
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− | + | ||
− | + | ||
− | == | + | ==2012 April 22== |
− | * | + | * Construct Galvos, Program Maple |
− | + | ||
− | + | ==2012 April 29== | |
− | + | * Write-up for Fluorescence Microscope | |
− | * | + | |
− | + | ||
− | == | + | ==2012 May 06== |
− | * | + | * |
Latest revision as of 06:53, 7 May 2012
Contents
Confocal Microscopy
Literature
- Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
- Confocal microscopy for biologists / Alan R. Hibbs.
- Handbook of biological confocal microscopy / editor, James B. Pawley.
Goals and Checkpoints
Goals
Key Factors | Proposed Technical Targets | Requirements for Success | Current Practice | Associated Technical Barriers |
---|---|---|---|---|
OCLSM Cost | < $8,000 | < $15,000 | > $100,000 commercial; ~$30,000 DIY | Cheap replacement for PMT & Galvo, etc. |
OCLSM Scan Time | 1 frame/second | 1 frame / 10 seconds | 10-50 fps | Fast scanning mirrors + ADC time |
Timeline
- 2 Weeks: Brightfield Confocal Microscopy
- 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
- 3 Weeks: Laser Scanning Features
Proposed Design
Challenges
- Control software
- Getting good resolution
Weekly Updates
2012 April 8
- Look for PMTs and/or Galvos
2012 April 15
- Calculations for stuff
2012 April 22
- Construct Galvos, Program Maple
2012 April 29
- Write-up for Fluorescence Microscope