Difference between revisions of "Assignment 5 Overview"
From Course Wiki
Juliesutton (Talk | contribs) (→Assignment details) |
Juliesutton (Talk | contribs) (→Assignment details) |
||
Line 28: | Line 28: | ||
#* Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.) | #* Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.) | ||
# Analysis and Results | # Analysis and Results | ||
− | #* Plot the MSD vs time (from the difference trajectories) for untreated and Cyto D treated cells on a single set of log-log axes. | + | #* Plot the MSD vs time (from the difference trajectories) for untreated and Cyto D treated cells on a single set of log-log axes. Plot the individual MSDs from each pair of beads in each movie. Use a single color for all the untreated cells, and a different color for all the treated cells. Do not include MSDs of particles not endocytosed by the cells. |
+ | #* Combine your data with others from the class to increase your sample size. | ||
# Discussion | # Discussion | ||
#* What kind of motion do you see described by your MSD vs τ results? | #* What kind of motion do you see described by your MSD vs τ results? |
Latest revision as of 13:34, 23 March 2020
Assignment details
This assignment has 2 parts:
- Part 1: Implementing difference tracking and measuring the stability of your microscope;
- Part 2: Live cell particle tracking of endocytosed beads.
Submit your work in on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment5.pdf.
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
- Overview
- Part 1: MSD difference tracking and microscope stability
- Part 2: Live cell particle tracking of endocytosed beads
Back to 20.309 Main Page