Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"

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===OD562 absorbance measurements for protein concentration calculations===
 
===OD562 absorbance measurements for protein concentration calculations===
−
During the 1 hour incubation of Part4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data [[Media:SampleData_microBCA.xlsx| '''here'''.]] You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical [[Media: How to use a protein standard curve.pdf | bulletin]] helpful.
+
During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data [[Media:SampleData_microBCA.xlsx| '''here'''.]] You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical [[Media: How to use a protein standard curve.pdf | bulletin]] helpful.
−
====T/R red team====
+
====T/R Class Data====
 +
TR Class microBCA assay data [[Media:TR_microBCA.xlsx | '''here'''.]]
 +
 
 +
====<font color="red">W/F red team</font color>====
 
{| border="1"
 
{| border="1"
 
|'''name'''
 
|'''name'''
Line 29: Line 32:
 
|0.054
 
|0.054
 
|-
 
|-
−
|I
+
|H
 
|0.034
 
|0.034
 
|-
 
|-
Line 39: Line 42:
 
|mutant
 
|mutant
 
|0.201
 
|0.201
 +
|}
 +
 +
====<font color=#FF6600>W/F orange team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|2.970
 +
|-
 +
|B
 +
|0.672
 +
|-
 +
|C
 +
|0.337
 +
|-
 +
|D
 +
|0.174
 +
|-
 +
|E
 +
|0.086
 +
|-
 +
|F
 +
|0.037
 +
|-
 +
|G
 +
|0.018
 +
|-
 +
|H
 +
|0.000
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.028
 +
|-
 +
|mutant
 +
|0.062
 +
|}
 +
 +
====<font color="blue">W/F blue team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|2.983
 +
|-
 +
|B
 +
|0.727
 +
|-
 +
|C
 +
|0.292
 +
|-
 +
|D
 +
|0.168
 +
|-
 +
|E
 +
|0.091
 +
|-
 +
|F
 +
|0.034
 +
|-
 +
|G
 +
|0.015
 +
|-
 +
|H
 +
|0.000
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.049
 +
|-
 +
|mutant
 +
|0.027
 +
|}
 +
 +
====<font color=#FF66CC>W/F pink team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|2.943
 +
|-
 +
|B
 +
|0.699
 +
|-
 +
|C
 +
|0.341
 +
|-
 +
|D
 +
|0.166
 +
|-
 +
|E
 +
|0.101
 +
|-
 +
|F
 +
|0.038
 +
|-
 +
|G
 +
|0.017
 +
|-
 +
|H
 +
|0.000
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.025
 +
|-
 +
|mutant
 +
|0.136
 +
|}
 +
 +
====<font color=#9933FF>W/F purple team</font color>====
 +
{| border="1"
 +
|'''name'''
 +
|'''OD562'''
 +
|-
 +
|colspan=2|standards
 +
|-
 +
|A
 +
|2.948
 +
|-
 +
|B
 +
|0.812
 +
|-
 +
|C
 +
|0.420
 +
|-
 +
|D
 +
|0.214
 +
|-
 +
|E
 +
|0.118
 +
|-
 +
|F
 +
|0.047
 +
|-
 +
|G
 +
|0.026
 +
|-
 +
|H
 +
|0.000
 +
|-
 +
|colspan=2| inverse pericam (IPC)
 +
|-
 +
|wild-type
 +
|0.016
 +
|-
 +
|mutant
 +
|0.010
 
|}
 
|}

Latest revision as of 20:49, 3 March 2016

OD562 absorbance measurements for protein concentration calculations

During the 1 hour incubation of Part 4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.

T/R Class Data

TR Class microBCA assay data here.

W/F red team

name OD562
standards
A 3.02
B 0.798
C 0.444
D 0.245
E 0.141
F 0.073
G 0.054
H 0.034
inverse pericam (IPC)
wild-type 0.177
mutant 0.201

W/F orange team

name OD562
standards
A 2.970
B 0.672
C 0.337
D 0.174
E 0.086
F 0.037
G 0.018
H 0.000
inverse pericam (IPC)
wild-type 0.028
mutant 0.062

W/F blue team

name OD562
standards
A 2.983
B 0.727
C 0.292
D 0.168
E 0.091
F 0.034
G 0.015
H 0.000
inverse pericam (IPC)
wild-type 0.049
mutant 0.027

W/F pink team

name OD562
standards
A 2.943
B 0.699
C 0.341
D 0.166
E 0.101
F 0.038
G 0.017
H 0.000
inverse pericam (IPC)
wild-type 0.025
mutant 0.136

W/F purple team

name OD562
standards
A 2.948
B 0.812
C 0.420
D 0.214
E 0.118
F 0.047
G 0.026
H 0.000
inverse pericam (IPC)
wild-type 0.016
mutant 0.010
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