Difference between revisions of "Talk:20.109(S16):Design mutation primers (Day2)"
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(→Primers for SDM) |
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Line 10: | Line 10: | ||
|5' CACCACAAAGagACTTGGCACCGTTATG 3' | |5' CACCACAAAGagACTTGGCACCGTTATG 3' | ||
|5' ATGGTGCCGTCCCCATCC 3' | |5' ATGGTGCCGTCCCCATCC 3' | ||
− | | | + | | E30R |
|- | |- | ||
|<font color = #FF9900>TR orange</font color> | |<font color = #FF9900>TR orange</font color> | ||
Line 20: | Line 20: | ||
|CACCACAAAGaAACTTGGCACCG | |CACCACAAAGaAACTTGGCACCG | ||
|ATGGTGCCGTCCCCATCC | |ATGGTGCCGTCCCCATCC | ||
− | | | + | | E30K |
|- | |- | ||
|<font color = #00FF00>TR green</font color> | |<font color = #00FF00>TR green</font color> | ||
Line 31: | Line 31: | ||
|5' CAAGGATGGGaACGGCACCAT 3' | |5' CAAGGATGGGaACGGCACCAT 3' | ||
|5' TCGAATAATGAGAAGGCTTCTTTG 3' | |5' TCGAATAATGAGAAGGCTTCTTTG 3' | ||
− | | | + | | D23N |
|- | |- | ||
|<font color = #FF99CC>TR pink</font color> | |<font color = #FF99CC>TR pink</font color> | ||
Line 44: | Line 44: | ||
|- | |- | ||
|<font color = #FF0000>WF red</font color> | |<font color = #FF0000>WF red</font color> | ||
− | |5' | + | |5' CAAGGATGGGcACGGCACCAT 3' |
− | |5' | + | |5' TCGAATAATGAGAAGGCTTCTTTG 3' |
− | | | + | | D23H |
|- | |- | ||
|<font color = #FF9900>WF orange</font color> | |<font color = #FF9900>WF orange</font color> | ||
− | |5' | + | |5'CCGT GTT TTT GAC gAG GAT GGG AAC 3' |
− | |5' | + | |5' AAT GCT TCT CGG ATT TCC TCT TCG C 3' |
− | | | + | |K93E |
|- | |- | ||
|<font color = #0000FF>WF blue</font color> | |<font color = #0000FF>WF blue</font color> | ||
|5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | |5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | ||
|5' CCATCAGCATCGACTTCATTG 3' | |5' CCATCAGCATCGACTTCATTG 3' | ||
− | | | + | |I62D |
|- | |- | ||
|<font color = #FF99CC>WF pink</font color> | |<font color = #FF99CC>WF pink</font color> | ||
− | |5' | + | |5' AAT GAA GTC GCT GCT GAT GGC 3' |
− | |5' | + | |5' GAT CAT ATC CTG CAA TTC TG 3' |
− | | | + | |D55A |
|- | |- | ||
|<font color = #CC00FF>WF purple</font color> | |<font color = #CC00FF>WF purple</font color> | ||
|5' CAAGGATGGGcACGGCACCAT 3' | |5' CAAGGATGGGcACGGCACCAT 3' | ||
|5' TCGAATAATGAGAAGGCTTCTTTG 3' | |5' TCGAATAATGAGAAGGCTTCTTTG 3' | ||
− | | | + | |D23H |
|} | |} |
Latest revision as of 15:20, 25 February 2016
Primers for SDM
Team | Forward primer | Reverse primer | Mutation (X#Z in CaM) | |
TR red | 5' CACCACAAAGagACTTGGCACCGTTATG 3' | 5' ATGGTGCCGTCCCCATCC 3' | E30R | |
TR orange | 5' ATTCGACAAGcatGGGGACGGCA 3' | 5' AATGAGAAGGCTTCTTTGAACTCTG 3' | D21H | |
TR yellow | CACCACAAAGaAACTTGGCACCG | ATGGTGCCGTCCCCATCC | E30K | |
TR green | 5' TTCGACAAGGgTGGGGACGGC 3' | 5' TAATGAGAAGGCTTCTTTGAACTCTGC 3' | D62G | |
TR blue | 5' CAAGGATGGGaACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23N | |
TR pink | 5' GTT GAT GAA ATG ATA AGG GAA cCA GAT ATC GAT GGT GAT GGC C 3' | 5' TTC TTC ATC TGT TAA CTT CTC CCC GAG GTT TGT CAT 3' | A127P | |
TR purple | 5' TTTTGACAAGcATGGGAACGGC 3' | 5' ACACGGAATGCTTCTCGG 3' | D94H | |
WF red | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23H | |
WF orange | 5'CCGT GTT TTT GAC gAG GAT GGG AAC 3' | 5' AAT GCT TCT CGG ATT TCC TCT TCG C 3' | K93E | |
WF blue | 5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | 5' CCATCAGCATCGACTTCATTG 3' | I62D | |
WF pink | 5' AAT GAA GTC GCT GCT GAT GGC 3' | 5' GAT CAT ATC CTG CAA TTC TG 3' | D55A | |
WF purple | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23H |