Difference between revisions of "20.109(F09): Mod 3 Day 2 Phage nanowires"
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=<center>Phage nanowires</center>= | =<center>Phage nanowires</center>= | ||
==Introduction== | ==Introduction== | ||
− | Edited from material originally written by the Belcher lab. Special thanks to Mark Allen, John Burpo. <br> | + | Edited from material originally written by the Belcher lab. Special thanks to Mark Allen, and Lieutenant Colonel F. John Burpo. <br> |
− | The materials prepared in this lab have activity as battery electrodes. The redox properties of the material will determine the operating voltage of the electrode, while other properties of the material will improve capacity (how long the battery will last under a given current load) and rate capability (how quickly the battery can be discharged or charged). Capacity and rate capability can be improved by either making materials very small (nanomaterials) or by incorporating conducting metals into the matrix of the material. | + | [[Image:CoinBattery.jpg|thumb|Coin-type battery]]The materials prepared in this lab have activity as battery electrodes. The redox properties of the material will determine the operating voltage of the electrode, while other properties of the material will improve capacity (how long the battery will last under a given current load) and rate capability (how quickly the battery can be discharged or charged). Capacity and rate capability can be improved by either making materials very small (nanomaterials) or by incorporating conducting metals into the matrix of the material. |
− | + | Assembly and testing of the phage-based battery will take place over several sessions. Today's lab will focus on material synthesis. The next lab will be analyzing material by TEM. In the final sessions of the module, dried materials will be formed into electrodes, and finally, these materials will be assembled into a coin type battery and tested. Groups will vary the amount of silver in the gold phage nanowires to see how these variations affect the charge/discharge cycle. | |
− | Assembly and testing of the phage-based battery will take place over several sessions. Today's lab will focus on material synthesis. The next lab will be analyzing material by TEM. In the final sessions of the module, dried materials will be formed into electrodes, and finally, these materials will be assembled into a coin type battery and tested. | + | Today in lab you will calculate the concentration of phage stock you've prepared and then react it with gold and silver. You will have time while these reactions are going on to work on the research proposal idea you've got started with your lab partner. |
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==Protocols== | ==Protocols== | ||
===Part 1: Dilute the 8#9 phage stock=== | ===Part 1: Dilute the 8#9 phage stock=== | ||
− | Count the number of plaques on your phage titering plates from last time. Calculate the concentration of phage in your undiluted purified phage sample. Be sure to take into account each dilution when you try this calculation. Express the concentration as PFU/ul and determine the volume of phage needed to make 10 ml of 3.5x10^7 PFU/ | + | Count the number of plaques on your phage titering plates from last time. Calculate the concentration of phage in your undiluted purified phage sample. Be sure to take into account each dilution when you try this calculation. Express the concentration as PFU/ul and determine the volume of phage needed to make 10 ml of 3.5x10^7 PFU/ |