Difference between revisions of "20.109(S24):Class data"
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<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> | <div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> | ||
{{Template:20.109(S24)}} | {{Template:20.109(S24)}} | ||
− | |||
− | |||
==Module 1: Drug discovery== | ==Module 1: Drug discovery== | ||
Line 10: | Line 8: | ||
Please find the SMM screen results [[Media:Screen results.csv | here]]. | Please find the SMM screen results [[Media:Screen results.csv | here]]. | ||
+ | |||
+ | |||
+ | Please find the EMSA results [https://www.dropbox.com/scl/fo/zd7u15wwjmscxzrnt9hkp/h?rlkey=5k8nwwpg80apvurbk5y1746eh&dl=0 here]. | ||
Line 26: | Line 27: | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 45.57 |
− | | | + | | 45.57 |
− | | | + | | 47.10 |
− | | | + | | 46.08 |
|- | |- | ||
| Small molecule #1 | | Small molecule #1 | ||
− | | | + | | 45.57 |
− | | | + | | 35.38 |
− | | | + | | 45.57 |
− | | | + | | 42.17 |
|- | |- | ||
| Small molecule #6 | | Small molecule #6 | ||
− | | | + | | 44.55 |
− | | | + | | 46.08 |
− | | | + | | 45.06 |
− | | | + | | 45.23 |
|- | |- | ||
| Small molecule #9 | | Small molecule #9 | ||
− | | | + | | 45.06 |
− | | | + | | 46.59 |
− | | | + | | 46.08 |
− | | | + | | 45.91 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 43.53 |
− | | | + | | 45.06 |
− | | | + | | 45.57 |
− | | | + | | 44.72 |
|- | |- | ||
| '''TR Yellow''' | | '''TR Yellow''' | ||
Line 76: | Line 77: | ||
|- | |- | ||
| Small molecule #11 | | Small molecule #11 | ||
− | | 44. | + | | 44.55 |
− | | 45. | + | | 45.57 |
− | | 44. | + | | 44.55 |
− | | 44. | + | | 44.89 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
− | | 47. | + | | 47.10 |
− | | 47. | + | | 47.10 |
− | | 49. | + | | 49.13 |
− | | 47. | + | | 47.78 |
|- | |- | ||
| '''TR Green''' | | '''TR Green''' | ||
Line 122: | Line 123: | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | 47. | + | | 47.11 |
− | | 45. | + | | 45.08 |
− | | 44. | + | | 44.06 |
− | | 45. | + | | 45.42 |
|- | |- | ||
| Small molecule #1 | | Small molecule #1 | ||
− | | 48. | + | | 48.64 |
− | | 48. | + | | 48.64 |
− | | 44. | + | | 44.06 |
− | | 47. | + | | 47.11 |
|- | |- | ||
| Small molecule #2 | | Small molecule #2 | ||
− | | 46. | + | | 46.60 |
− | | 47. | + | | 47.11 |
− | | 43. | + | | 43.56 |
− | | 45. | + | | 45.76 |
|- | |- | ||
| Small molecule #4 | | Small molecule #4 | ||
− | | 46. | + | | 46.60 |
− | | 44. | + | | 44.57 |
− | | 43. | + | | 43.56 |
− | | 44. | + | | 44.91 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | 47. | + | | 47.62 |
− | | 44. | + | | 44.57 |
− | | 44. | + | | 44.06 |
− | | 45. | + | | 45.42 |
|- | |- | ||
| '''TR Pink''' | | '''TR Pink''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 47.11 |
− | | | + | | 44.57 |
− | | | + | | 44.57 |
− | | | + | | 45.42 |
|- | |- | ||
| Small molecule #1 | | Small molecule #1 | ||
− | | | + | | 46.10 |
− | | | + | | 46.60 |
− | | | + | | 45.08 |
− | | | + | | 45.93 |
|- | |- | ||
| Small molecule #2 | | Small molecule #2 | ||
− | | | + | | 45.59 |
− | | | + | | 46.10 |
− | | | + | | 47.62 |
− | | | + | | 46.43 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 47.11 |
− | | | + | | 44.06 |
− | | | + | | 45.57 |
− | | | + | | 45.25 |
|- | |- | ||
| Small molecule #11 | | Small molecule #11 | ||
− | | | + | | 45.08 |
− | | | + | | 44.06 |
− | | | + | | 44.06 |
− | | | + | | 44.40 |
|- | |- | ||
|} | |} | ||
Line 199: | Line 200: | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 47.05 |
− | | | + | | 44.51 |
− | | | + | | 35.86 |
− | | | + | | 42.47 |
|- | |- | ||
| Small molecule #3 | | Small molecule #3 | ||
− | | | + | | 45.53 |
− | | | + | | 46.55 |
− | | | + | | 46.04 |
− | | | + | | 46.04 |
|- | |- | ||
| Small molecule #4 | | Small molecule #4 | ||
− | | | + | | 46.55 |
− | | | + | | 47.05 |
− | | | + | | 45.53 |
− | | | + | | 46.38 |
|- | |- | ||
| Small molecule #5 | | Small molecule #5 | ||
− | | | + | | 43.49 |
− | | | + | | 47.06 |
− | | | + | | 46.55 |
− | | | + | | 45.70 |
|- | |- | ||
| Small molecule #8 | | Small molecule #8 | ||
− | | | + | | 45.02 |
− | | | + | | 46.55 |
− | | | + | | 45.53 |
− | | | + | | 45.70 |
|- | |- | ||
| '''WF Orange''' | | '''WF Orange''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 44.02 |
− | | | + | | 45.55 |
− | | | + | | 46.05 |
− | | | + | | 45.21 |
|- | |- | ||
| Small molecule #1 | | Small molecule #1 | ||
− | | | + | | 43.09 |
− | | | + | | 46.56 |
− | | | + | | 46.56 |
− | | | + | | 45.40 |
|- | |- | ||
| Small molecule #2 | | Small molecule #2 | ||
− | | | + | | 44.53 |
− | | | + | | 45.04 |
− | | | + | | 44.53 |
− | | | + | | 44.77 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 45.04 |
− | | | + | | 46.56 |
− | | | + | | 45.04 |
− | | | + | | 45.55 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
− | | | + | | 46.05 |
− | | | + | | 44.53 |
− | | | + | | 44.53 |
− | | | + | | 45.04 |
|- | |- | ||
| '''WF Yellow''' | | '''WF Yellow''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 43.50 |
− | | | + | | 46.04 |
− | | | + | | 45.53 |
− | | | + | | 45.02 |
|- | |- | ||
| Small molecule #2 | | Small molecule #2 | ||
− | | | + | | 44.52 |
− | | | + | | 46.04 |
− | | | + | | 45.53 |
− | | | + | | 45.36 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 44.52 |
− | | | + | | 46.04 |
− | | | + | | 48.59 |
− | | | + | | 46.38 |
|- | |- | ||
| Small molecule #11 | | Small molecule #11 | ||
− | | | + | | 46.04 |
− | | | + | | 45.02 |
− | | | + | | 47.57 |
− | | | + | | 46.21 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
− | | | + | | 44.01 |
− | | | + | | 43.50 |
− | | | + | | 45.02 |
− | | | + | | 44.18 |
|- | |- | ||
| '''WF Green''' | | '''WF Green''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 45.04 |
− | | | + | | 43.51 |
− | | | + | | 43.00 |
− | | | + | | 43.85 |
|- | |- | ||
| Small molecule #2 | | Small molecule #2 | ||
− | | | + | | 46.05 |
− | | | + | | 46.05 |
− | | | + | | 46.56 |
− | | | + | | 46.22 |
|- | |- | ||
| Small molecule #9 | | Small molecule #9 | ||
− | | | + | | 46.05 |
− | | | + | | 45.04 |
− | | | + | | 46.05 |
− | | | + | | 45.71 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 45.04 |
− | | | + | | 45.04 |
− | | | + | | 45.04 |
− | | | + | | 45.04 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
− | | | + | | 44.53 |
− | | | + | | 47.07 |
− | | | + | | 44.54 |
− | | | + | | 45.38 |
|- | |- | ||
| '''WF Blue''' | | '''WF Blue''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 46.04 |
− | | | + | | 44.52 |
− | | | + | | 43.50 |
− | | | + | | 44.685 |
|- | |- | ||
| Small molecule #5 | | Small molecule #5 | ||
− | | | + | | 45.02 |
− | | | + | | 43.50 |
− | | | + | | 45.02 |
− | | | + | | 45.70 |
|- | |- | ||
| Small molecule #9 | | Small molecule #9 | ||
− | | | + | | 47.06 |
− | | | + | | 47.06 |
− | | | + | | 45.02 |
− | | | + | | 46.38 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 48.08 |
− | | | + | | 45.02 |
− | | | + | | 44.01 |
− | | | + | | 45.70 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
− | | | + | | 45.53 |
− | | | + | | 43.50 |
− | | | + | | 44.01 |
− | | | + | | 44.35 |
|- | |- | ||
| '''WF Pink''' | | '''WF Pink''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 44.01819 |
− | | | + | | 46.46263 |
− | | | + | | 45.54488 |
− | | | + | | 45.3419 |
|- | |- | ||
| Small molecule #5 | | Small molecule #5 | ||
− | | | + | | 46.05378 |
− | | | + | | 45.03598 |
− | | | + | | 47.58047 |
− | | | + | | 46.22341 |
|- | |- | ||
| Small molecule #9 | | Small molecule #9 | ||
− | | | + | | 45.03598 |
− | | | + | | 46.56268 |
− | | | + | | 45.54488 |
− | | | + | | 45.71451 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 44.01819 |
− | | | + | | 46.56268 |
− | | | + | | 44.52709 |
− | | | + | | 45.0360 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
− | | | + | | 44.01819 |
− | | | + | | 44.52709 |
− | | | + | | 46.05378 |
− | | | + | | 44.86635 |
|- | |- | ||
| '''WF Purple''' | | '''WF Purple''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 47.05 |
− | | | + | | 43.50 |
− | | | + | | 42.99 |
− | | | + | | 44.51 |
|- | |- | ||
| Small molecule #5 | | Small molecule #5 | ||
− | | | + | | 45.53 |
− | | | + | | 47.06 |
− | | | + | | 45.53 |
− | | | + | | 46.04 |
|- | |- | ||
| Small molecule #9 | | Small molecule #9 | ||
− | | | + | | 42.98 |
− | | | + | | 46.04 |
− | | | + | | 48.59 |
− | | | + | | 45.87 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 44.51 |
− | | | + | | 45.02 |
− | | | + | | 46.55 |
− | | | + | | 45.36 |
|- | |- | ||
| Small molecule #13 | | Small molecule #13 | ||
+ | | 46.04 | ||
+ | | 43.50 | ||
+ | | 46.04 | ||
+ | | 45.19 | ||
+ | |- | ||
+ | |- | ||
+ | |} | ||
+ | </center> | ||
+ | |||
+ | |||
+ | <center> | ||
+ | {| border="1" | ||
+ | |- | ||
| | | | ||
− | | | + | | Replicate #1 |
− | | | + | | Replicate #2 |
− | | | + | | Replicate #3 |
+ | | Average T<sub>m</sub> | ||
|- | |- | ||
| '''WF Teal''' | | '''WF Teal''' | ||
|- | |- | ||
| DMSO | | DMSO | ||
− | | | + | | 45.54 |
− | | | + | | 45.54 |
− | | | + | |43.00 |
− | | | + | | 44.69 |
|- | |- | ||
| Small molecule #1 | | Small molecule #1 | ||
− | | | + | | 47.07 |
− | | | + | | 46.05 |
− | | | + | | 44.53 |
− | | | + | | 45.88 |
|- | |- | ||
| Small molecule #2 | | Small molecule #2 | ||
− | | | + | | 44.53 |
− | | | + | | 45.04 |
− | | | + | | 45.54 |
− | | | + | | 45.04 |
|- | |- | ||
| Small molecule #10 | | Small molecule #10 | ||
− | | | + | | 46.56 |
− | | | + | | 46.56 |
− | | | + | | 47.07 |
− | | | + | | 46.73 |
|- | |- | ||
| Small molecule #11 | | Small molecule #11 | ||
− | | | + | | 47.07 |
− | | | + | | 46.56 |
− | | | + | | 44.53 |
− | | | + | | 46.05 |
|- | |- | ||
|} | |} | ||
Line 457: | Line 472: | ||
==Module 2: Protein engineering== | ==Module 2: Protein engineering== | ||
− | Please find uploaded data for Mod 2 in the Dropbox folder linked [[ here]]. | + | Please find uploaded data for Mod 2 in the Dropbox folder linked [[https://www.dropbox.com/scl/fo/pfoqp6ndpzt3kt6kbgbdv/h?rlkey=6sp3sw3rds01y2xv9qyqc9t2o&dl=0 here]]. |
+ | |||
+ | ====T/R==== | ||
+ | |||
+ | <center> | ||
+ | {| border=1px | ||
+ | |'''Team''' | ||
+ | |'''Peptide sequence''' | ||
+ | |'''DNA sequence for peptide''' | ||
+ | |- | ||
+ | |TR orange | ||
+ | | DCCDCC | ||
+ | | GAC TGT TGC GAT TGT TGC | ||
+ | |- | ||
+ | |TR yellow | ||
+ | | CCGCCGCC | ||
+ | | TGC TGT GGC TGT TGT GGC TGT TGC | ||
+ | |- | ||
+ | |TR green | ||
+ | | CGCGGHGH | ||
+ | | TGC GGA TGT GGA GGT CAT GGT CAC | ||
+ | |- | ||
+ | |TR blue | ||
+ | | GCCGCCG | ||
+ | | GGA TGT TGT GGA TGC TGT GGC | ||
+ | |- | ||
+ | |TR pink | ||
+ | | GCHGCH | ||
+ | | GGT TGT CAC GGT TGT CAC | ||
+ | |} | ||
+ | </center> | ||
+ | |||
+ | ====W/F==== | ||
+ | |||
+ | <center> | ||
+ | {| border=1px | ||
+ | |'''Team''' | ||
+ | |'''Peptide sequence''' | ||
+ | |'''DNA sequence for peptide''' | ||
+ | |- | ||
+ | |WF red | ||
+ | | GHGDGHGD | ||
+ | | GGT CAC GGT GAC GGT CAC GGT GAC | ||
+ | |- | ||
+ | |WF orange | ||
+ | | CDCCDCCD | ||
+ | | TGT GAC TGT TGC GAC TGC TGC GAC | ||
+ | |- | ||
+ | |WF yellow | ||
+ | | CGHCGCC | ||
+ | | TGT GGT CAT TGT GGT TGT TGT | ||
+ | |- | ||
+ | |WF green | ||
+ | | HEHEHE | ||
+ | | CAC GAA CAC GAA CAC GAA | ||
+ | |- | ||
+ | |WF blue | ||
+ | | PCACACA | ||
+ | | CCA TGT GCA TGT GCA TGT GCC | ||
+ | |- | ||
+ | |WF teal | ||
+ | | GHHGHH | ||
+ | | GGA CAT CAC GGA CAT CAC | ||
+ | |- | ||
+ | |WF pink | ||
+ | | GCHDWC | ||
+ | | GGA TGT CAT GAT TGG TGC | ||
+ | |- | ||
+ | |WF purple | ||
+ | | DCCDCCD | ||
+ | | GAC TGT TGC GAT TGC TGT GAC | ||
+ | |} | ||
+ | </center> |
Latest revision as of 17:51, 10 April 2024
Module 1: Drug discovery
Please find uploaded data for Mod 1 in the Dropbox folder linked here.
Please find the SMM screen results here.
Please find the EMSA results here.
DSF results:
Replicate #1 | Replicate #2 | Replicate #3 | Average Tm | |
TR Orange | ||||
DMSO | 45.57 | 45.57 | 47.10 | 46.08 |
Small molecule #1 | 45.57 | 35.38 | 45.57 | 42.17 |
Small molecule #6 | 44.55 | 46.08 | 45.06 | 45.23 |
Small molecule #9 | 45.06 | 46.59 | 46.08 | 45.91 |
Small molecule #10 | 43.53 | 45.06 | 45.57 | 44.72 |
TR Yellow | ||||
DMSO | 48.12 | 47.61 | 49.13 | 48.29 |
Small molecule #2 | 48.62 | 46.59 | 44.55 | 46.59 |
Small molecule #10 | 47.61 | 47.61 | 47.61 | 47.61 |
Small molecule #11 | 44.55 | 45.57 | 44.55 | 44.89 |
Small molecule #13 | 47.10 | 47.10 | 49.13 | 47.78 |
TR Green | ||||
DMSO | 43.02 | 45.06 | 45.06 | 44.37 |
Small molecule #2 | 43.02 | 47.10 | 45.57 | 45.23 |
Small molecule #6 | 46.08 | 49.65 | 46.08 | 47.27 |
Small molecule #9 | 44.04 | 45.06 | 46.59 | 45.23 |
Small molecule #10 | 46.08 | 45.57 | 48.62 | 46.76 |
TR Blue | ||||
DMSO | 47.11 | 45.08 | 44.06 | 45.42 |
Small molecule #1 | 48.64 | 48.64 | 44.06 | 47.11 |
Small molecule #2 | 46.60 | 47.11 | 43.56 | 45.76 |
Small molecule #4 | 46.60 | 44.57 | 43.56 | 44.91 |
Small molecule #10 | 47.62 | 44.57 | 44.06 | 45.42 |
TR Pink | ||||
DMSO | 47.11 | 44.57 | 44.57 | 45.42 |
Small molecule #1 | 46.10 | 46.60 | 45.08 | 45.93 |
Small molecule #2 | 45.59 | 46.10 | 47.62 | 46.43 |
Small molecule #10 | 47.11 | 44.06 | 45.57 | 45.25 |
Small molecule #11 | 45.08 | 44.06 | 44.06 | 44.40 |
Replicate #1 | Replicate #2 | Replicate #3 | Average Tm | |
WF Red | ||||
DMSO | 47.05 | 44.51 | 35.86 | 42.47 |
Small molecule #3 | 45.53 | 46.55 | 46.04 | 46.04 |
Small molecule #4 | 46.55 | 47.05 | 45.53 | 46.38 |
Small molecule #5 | 43.49 | 47.06 | 46.55 | 45.70 |
Small molecule #8 | 45.02 | 46.55 | 45.53 | 45.70 |
WF Orange | ||||
DMSO | 44.02 | 45.55 | 46.05 | 45.21 |
Small molecule #1 | 43.09 | 46.56 | 46.56 | 45.40 |
Small molecule #2 | 44.53 | 45.04 | 44.53 | 44.77 |
Small molecule #10 | 45.04 | 46.56 | 45.04 | 45.55 |
Small molecule #13 | 46.05 | 44.53 | 44.53 | 45.04 |
WF Yellow | ||||
DMSO | 43.50 | 46.04 | 45.53 | 45.02 |
Small molecule #2 | 44.52 | 46.04 | 45.53 | 45.36 |
Small molecule #10 | 44.52 | 46.04 | 48.59 | 46.38 |
Small molecule #11 | 46.04 | 45.02 | 47.57 | 46.21 |
Small molecule #13 | 44.01 | 43.50 | 45.02 | 44.18 |
WF Green | ||||
DMSO | 45.04 | 43.51 | 43.00 | 43.85 |
Small molecule #2 | 46.05 | 46.05 | 46.56 | 46.22 |
Small molecule #9 | 46.05 | 45.04 | 46.05 | 45.71 |
Small molecule #10 | 45.04 | 45.04 | 45.04 | 45.04 |
Small molecule #13 | 44.53 | 47.07 | 44.54 | 45.38 |
WF Blue | ||||
DMSO | 46.04 | 44.52 | 43.50 | 44.685 |
Small molecule #5 | 45.02 | 43.50 | 45.02 | 45.70 |
Small molecule #9 | 47.06 | 47.06 | 45.02 | 46.38 |
Small molecule #10 | 48.08 | 45.02 | 44.01 | 45.70 |
Small molecule #13 | 45.53 | 43.50 | 44.01 | 44.35 |
WF Pink | ||||
DMSO | 44.01819 | 46.46263 | 45.54488 | 45.3419 |
Small molecule #5 | 46.05378 | 45.03598 | 47.58047 | 46.22341 |
Small molecule #9 | 45.03598 | 46.56268 | 45.54488 | 45.71451 |
Small molecule #10 | 44.01819 | 46.56268 | 44.52709 | 45.0360 |
Small molecule #13 | 44.01819 | 44.52709 | 46.05378 | 44.86635 |
WF Purple | ||||
DMSO | 47.05 | 43.50 | 42.99 | 44.51 |
Small molecule #5 | 45.53 | 47.06 | 45.53 | 46.04 |
Small molecule #9 | 42.98 | 46.04 | 48.59 | 45.87 |
Small molecule #10 | 44.51 | 45.02 | 46.55 | 45.36 |
Small molecule #13 | 46.04 | 43.50 | 46.04 | 45.19 |
Replicate #1 | Replicate #2 | Replicate #3 | Average Tm | |
WF Teal | ||||
DMSO | 45.54 | 45.54 | 43.00 | 44.69 |
Small molecule #1 | 47.07 | 46.05 | 44.53 | 45.88 |
Small molecule #2 | 44.53 | 45.04 | 45.54 | 45.04 |
Small molecule #10 | 46.56 | 46.56 | 47.07 | 46.73 |
Small molecule #11 | 47.07 | 46.56 | 44.53 | 46.05 |
Module 2: Protein engineering
Please find uploaded data for Mod 2 in the Dropbox folder linked [here].
T/R
Team | Peptide sequence | DNA sequence for peptide |
TR orange | DCCDCC | GAC TGT TGC GAT TGT TGC |
TR yellow | CCGCCGCC | TGC TGT GGC TGT TGT GGC TGT TGC |
TR green | CGCGGHGH | TGC GGA TGT GGA GGT CAT GGT CAC |
TR blue | GCCGCCG | GGA TGT TGT GGA TGC TGT GGC |
TR pink | GCHGCH | GGT TGT CAC GGT TGT CAC |
W/F
Team | Peptide sequence | DNA sequence for peptide |
WF red | GHGDGHGD | GGT CAC GGT GAC GGT CAC GGT GAC |
WF orange | CDCCDCCD | TGT GAC TGT TGC GAC TGC TGC GAC |
WF yellow | CGHCGCC | TGT GGT CAT TGT GGT TGT TGT |
WF green | HEHEHE | CAC GAA CAC GAA CAC GAA |
WF blue | PCACACA | CCA TGT GCA TGT GCA TGT GCC |
WF teal | GHHGHH | GGA CAT CAC GGA CAT CAC |
WF pink | GCHDWC | GGA TGT CAT GAT TGG TGC |
WF purple | DCCDCCD | GAC TGT TGC GAT TGC TGT GAC |