Difference between revisions of "20.109(S22):Spring 2022 schedule"
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| T/W Mar 8/9 | | T/W Mar 8/9 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D1 lecture slides.pdf|Lecture slides]] | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D1 lecture slides.pdf|Lecture slides]] | ||
− | | [[20.109(S22):M2D1 |Complete in-silico cloning of pdCas9 expression plasmid]] <br> | + | | [[20.109(S22):M2D1 |Complete in-silico cloning of pdCas9 expression plasmid]] <br> [[Media:Sp22 M2D1 bcm marked.pdf | T/R prelab]] <br> [[Media:Sp22_M2D1_jz.pdf | W/R prelab]] |
| [[20.109(S22):Homework#Due_M2D1|Homework due]] | | [[20.109(S22):Homework#Due_M2D1|Homework due]] | ||
|-- | |-- | ||
| M2D2 | | M2D2 | ||
| R/F Mar 10/11 | | R/F Mar 10/11 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D2 lecture slides.pdf|Lecture slides]] |
− | | [[20.109(S22):M2D2 |Design gRNA sequence for CRIPSRi system]] <br> | + | | [[20.109(S22):M2D2 |Design gRNA sequence for CRIPSRi system]] <br> [[Media:SP22 M2D2 bcm marked.pdf |T/R prelab]] <br> [[Media:SP22 M2D2 jz.pdf | W/F prelab]] |
| [[20.109(S22):Homework#Due_M2D2| Homework due]] <br>[[20.109(S22):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sat, Mar 12 at 10 pm <br> | | [[20.109(S22):Homework#Due_M2D2| Homework due]] <br>[[20.109(S22):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sat, Mar 12 at 10 pm <br> | ||
|-- | |-- | ||
| M2D3 | | M2D3 | ||
| T/W Mar 15/16 | | T/W Mar 15/16 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D3 lecture slides.pdf|Lecture slides]] |
− | | [[20.109(S22):M2D3 |Clone psgRNA expression plasmid]] <br> | + | | [[20.109(S22):M2D3 |Clone psgRNA expression plasmid]] <br> [[Media:Sp22 M2D3 bcm marked.pdf | T/R prelab]] <br> [[Media: Sp22_M2D3_jz_pdf.pdf | W/F prelab]] |
| [[20.109(S22):Homework#Due_M2D3|Homework due]]<br>[https://app.slack.com/client/T01KU1VLZQB/C0323Q19V89 Blog post due] Mon, Mar 14 at 10 pm | | [[20.109(S22):Homework#Due_M2D3|Homework due]]<br>[https://app.slack.com/client/T01KU1VLZQB/C0323Q19V89 Blog post due] Mon, Mar 14 at 10 pm | ||
|-- | |-- | ||
| M2D4 | | M2D4 | ||
| R/F Mar 17/18 | | R/F Mar 17/18 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D4 lecture slides.pdf|Lecture slides]] |
− | | [[20.109(S22):M2D4 |Confirm gRNA sequence in psgRNA expression plasmid]] <br> | + | | [[20.109(S22):M2D4 |Confirm gRNA sequence in psgRNA expression plasmid]] <br> [[Media:Spring2022 M2D4 CZ v2.pdf | T/R Prelab]] [[Media:Sp22 JC presentation.pdf | JC Notes]] <br> [[Media:Spring2022 M2D4 CZ v2.pdf | W/F Prelab]] |
| <font color= #819081 >'''Laboratory quiz'''</font color> <br> [[20.109(S22):Homework#Due_M2D4|Homework due]]<br>[[20.109(S22):Data Summary| <font color = #b3251c>'''Data Summary revision due'''</font color>]] Sun, Mar 20 at 10 pm <br> | | <font color= #819081 >'''Laboratory quiz'''</font color> <br> [[20.109(S22):Homework#Due_M2D4|Homework due]]<br>[[20.109(S22):Data Summary| <font color = #b3251c>'''Data Summary revision due'''</font color>]] Sun, Mar 20 at 10 pm <br> | ||
|-- | |-- | ||
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| M2D5 | | M2D5 | ||
| T/W Apr 5/6 | | T/W Apr 5/6 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D5 lecture slides.pdf|Lecture slides]] |
− | | [[20.109(S22):M2D5 |Prepare for induction of CRISPRi system]] <br> | + | | [[20.109(S22):M2D5 |Prepare for induction of CRISPRi system]] <br> [[Media:Sp22 M2D5 bcm marked.pdf | T/R prelab]] <br> [[Media:Sp22 M2D5 jz.pdf | W/F prelab]] |
| [[20.109(S22):Homework#Due_M2D5|Homework due]] | | [[20.109(S22):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
| M2D6 | | M2D6 | ||
| R/F Apr 7/8 | | R/F Apr 7/8 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D6 lecture slides.pdf|Lecture slides]] |
− | | [[20.109(S22):M2D6 |Complete CRISPRi experiment and measure fermentation products]] <br> | + | | [[20.109(S22):M2D6 |Complete CRISPRi experiment and measure fermentation products]] <br> [[Media:Sp22 M2D6 bcm marked.pdf | T/R prelab]]<br> [[Media:Sp22_M2D6_jz.pdf | W/F prelab ]] |
− | | [[20.109(S22):Homework#Due_M2D6|Homework due]]<br> | + | | [[20.109(S22):Homework#Due_M2D6|Homework due]]<br> |
|-- | |-- | ||
| M2D7 | | M2D7 | ||
| T/W Apr 12/13 | | T/W Apr 12/13 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 M2D7 lecture slides.pdf|Lecture slides]] |
− | | [[20.109(S22):M2D7 |Examine features in gRNA-targeted genomic sequences]] <br> | + | | [[20.109(S22):M2D7 |Examine features in gRNA-targeted genomic sequences]] <br> [[Media:Sp22 M2D7 bcm marked.pdf | T/R prelab]] <br> [[Media:Sp22 M2D7 jz.pdf | W/F prelab]] |
− | | | + | | [[20.109(S22):Homework#Due_M2D7|Homework due]]<br> |
|-- | |-- | ||
| M2D8 | | M2D8 | ||
| R/F Apr 14/15 | | R/F Apr 14/15 | ||
| Comm Lab workshop <br> | | Comm Lab workshop <br> | ||
− | | [[20.109(S22):M2D8 |Organize figures and outline text for Research article]] <br> | + | | [[20.109(S22):M2D8 |Organize figures and outline text for Research article]] <br> [[Media:Sp22 RA discussion marked.pdf | prelab]] |
− | | [[20.109(S22):Homework#Due_M2D8|Homework due]] <br> | + | | <font color= #819081 >'''Laboratory quiz'''</font color> <br> [[20.109(S22):Homework#Due_M2D8|Homework due]] <br> |
|-- | |-- | ||
| colspan="6" | | | colspan="6" | | ||
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| M3D1 | | M3D1 | ||
| R/F Apr 21/22 | | R/F Apr 21/22 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [[Media:Sp22 Drug discovery ethics exercise.pdf|Lecture slides]] |
− | | [[20.109(S22):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> | + | | [[20.109(S22):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> [[Media:Sp22 M3D1 bcm.pdf | T/R prelab]] <br> [[Media:Sp22 M3D1 jz.pdf | W/F prelab]] |
| [[20.109(S22):Homework#Due_M3D1|Homework due]] <br> [[20.109(S22):Research article| <font color = #b3251c>'''Research article due'''</font color>]] Sat, Apr 23 at 10 pm | | [[20.109(S22):Homework#Due_M3D1|Homework due]] <br> [[20.109(S22):Research article| <font color = #b3251c>'''Research article due'''</font color>]] Sat, Apr 23 at 10 pm | ||
|-- | |-- | ||
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| T/W Apr 26/27 | | T/W Apr 26/27 | ||
| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
− | | <font color = #819081>'''Research proposal pitches'''</font color> | + | | <font color = #819081>'''Research proposal pitches'''</font color> <br> [[Media:Sp22 proposal pitches bcm.pdf | T/R prelab]] <br> [[Media:Sp22 proposal pitches jz_fixed.pdf | W/F prelab]] |
| <br> [https://app.slack.com/client/T01KU1VLZQB/C0323Q19V89 Blog post due] Mon, Apr 25 at 10 pm | | <br> [https://app.slack.com/client/T01KU1VLZQB/C0323Q19V89 Blog post due] Mon, Apr 25 at 10 pm | ||
|-- | |-- | ||
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| R/F Apr 28/29 | | R/F Apr 28/29 | ||
| Comm Lab workshop | | Comm Lab workshop | ||
− | | [[20.109(S22):M3D2 |Develop ideas for Research proposal presentation ]] <br> | + | | [[20.109(S22):M3D2 |Develop ideas for Research proposal presentation ]] <br> [[Media:Sp22 M3D2 bcm.pdf | T/R prelab]] <br> [[Media:Sp22 M3D2 jz.pdf | W/F prelab]] |
| [[20.109(S22):Homework#Due_M3D2|Homework due]]<br> | | [[20.109(S22):Homework#Due_M3D2|Homework due]]<br> | ||
|-- | |-- | ||
| M3D3 | | M3D3 | ||
| T/W May 3/4 | | T/W May 3/4 | ||
− | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp22 Ethics for research proposals.pdf| Lecture slides]] |
− | | [[20.109(S22):M3D3 |Participate in Research proposal peer reviews]] <br> | + | | [[20.109(S22):M3D3 |Participate in Research proposal peer reviews]] <br> [[Media:Sp22 M3D3 bcm.pdf | T/R prelab]] <br> [[Media:Sp22 M3D3 jz.pdf | W/F prelab]] |
| [[20.109(S22):Homework#Due_M3D3|Homework due]] | | [[20.109(S22):Homework#Due_M3D3|Homework due]] | ||
|-- | |-- |
Latest revision as of 16:41, 4 May 2022
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 1-390
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322
ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).