Difference between revisions of "20.109(S20): Spring 2020 schedule"
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| 8 | | 8 | ||
| T/W Apr 7/8 | | T/W Apr 7/8 | ||
− | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br>[https://www.google.com/url?q=https%3A%2F%2Fmit.zoom.us%2Fj%2F348659452&sa=D&ust=1585502144595000&usg=AFQjCNG0iLNO3f-D3L837sTrvXtzc-0Vtw Zoom access]<br>[ M2D8 video]<br>[[Media: | + | | [http://be.mit.edu/directory/ernest-fraenkel EF]<br>[https://www.google.com/url?q=https%3A%2F%2Fmit.zoom.us%2Fj%2F348659452&sa=D&ust=1585502144595000&usg=AFQjCNG0iLNO3f-D3L837sTrvXtzc-0Vtw Zoom access]<br>[https://mit.zoom.us/rec/share/7MVRHeGgxnxIUtbA1EPCZekTPonfT6a8hiUdrPALn0vGNKg00SKcZRoEF2p4I905 M2D8 video]<br>[[Media:Lecture 4 transcriptional regulation FINAL to post.pdf|M2D8 slides]] |
− | | [[20.109(S20):Examine qPCR results and complete data analysis (Day8) | Complete RNA-seq data analysis]]<br>[https://mit.zoom.us/s/613303093 T/R Zoom access][ T/R prelab video], [[Media: Sp20_M2D8_LMM.pdf | T/R prelab slides]]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | | [[20.109(S20):Examine qPCR results and complete data analysis (Day8) | Complete RNA-seq data analysis]]<br>[https://mit.zoom.us/s/613303093 T/R Zoom access],[https://mit.zoom.us/rec/share/4eNSHo7f6U5IUp31-HyEaLAxAbnuX6a8g3ce_aZcnhqsCwkfQw9Zcc7oyKPL4gnH?startTime=1586279524000 T/R prelab video], [[Media: Sp20_M2D8_LMM.pdf | T/R prelab slides]]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/38FbIoD3qntJTI3wuALwSP4FOL3Leaa8gXUbrvZeyUomVGcB1C_E7a6zsXa-GRJa W/F prelab video], [[Media:Sp20 M2D8 bcm unmarked.pdf| WF prelab slides]] |
| [[20.109(S20):Homework#Due_M2D8| Homework due]] | | [[20.109(S20):Homework#Due_M2D8| Homework due]] | ||
|-- | |-- | ||
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| 9 | | 9 | ||
| R/F Apr 9/10 | | R/F Apr 9/10 | ||
− | | BE Comm Lab<br> [https://mit.zoom.us/j/714801029 Zoom access] | + | | BE Comm Lab<br> [https://mit.zoom.us/j/714801029 Zoom access]<br>[https://mit.zoom.us/rec/share/-sFoLIrpyDpOf4mdyGHQeJdiMLW9T6a80SZN_PQPmhlGwu5gvM4N--6ZxpDuu6oO M2D9 video] |
− | | Prepare M2 Major assignments: Journal club presentation and Research article<br>[https://mit.zoom.us/j/137247921 T/R Zoom access]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | |Prepare M2 Major assignments: Journal club presentation and Research article<br>[https://mit.zoom.us/j/137247921 T/R Zoom access], [https://mit.zoom.us/rec/share/zMFfNLvUx35LQZXm81HEV7EgIKP7eaa82iIc86YEy0yWAbiNrz9HLzjA-hDoo1R4 T/R prelab video], [[Media:Sp20 M2D9Prelab nll.pdf|T/R prelab slides]]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/4_xXILPO2GdLWYXQsFzUCvYcE73rT6a8gCka_fQEzkbSJypqfq7CeIAspEvXlPKZ W/F prelab video], [[Media:Sp20 M2D9Prelab nll.pdf|W/F prelab slides]] |
| <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M2D9| Homework due]] <br> [[20.109(S20):Journal club presentations (Day4 and 5)|<font color=#B40404>'''Journal club presentation due'''</font color>]] Sat, Apr 11 at 10 pm <br>[https://be20109s20.blogspot.com/ Blog post due] Mon, Apr 13 at 10 pm <br> | | <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M2D9| Homework due]] <br> [[20.109(S20):Journal club presentations (Day4 and 5)|<font color=#B40404>'''Journal club presentation due'''</font color>]] Sat, Apr 11 at 10 pm <br>[https://be20109s20.blogspot.com/ Blog post due] Mon, Apr 13 at 10 pm <br> | ||
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| 1 | | 1 | ||
| T/W Apr 14/15 | | T/W Apr 14/15 | ||
− | | [http://be.mit.edu/directory/leslie-mcclain LM]<br> | + | | [http://be.mit.edu/directory/leslie-mcclain LM]<br>[https://mit.zoom.us/s/613303093 Zoom access]<br>[[Media:Sp20_Mod3_lecture 1.pdf|M3D1 slides]]<br>[https://mit.zoom.us/rec/share/_egpI63800VLSa_fxVz5ZK1iAbrUX6a81yMbrvtexUgATJB4EXCPChegA1vEGO9u?startTime=1586876971000 M3D1 video] |
− | | [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | | [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access], [https://mit.zoom.us/rec/share/4Z0oEY6s_WJJZ43S9UPvYKIfFKLvX6a80CNMrPJbzhlG5xmsEUZbnQpncq4-uB8Z T/R prelab video], [[Media:Sp20 M3D1 nll.pdf|T/R prelab slides]]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/w-xIM4383W5OT9LXxmPeYvAeWYTqX6a81SAf8vFfmBq0uu3N_aBsQAOdLlVV3XbI W/F prelab video], [[Media:Sp20 M3D1 bcm unmarked.pdf | WF prelab slides]] |
| [[20.109(S20):Homework#Due_M3D1| Homework due]] <br> | | [[20.109(S20):Homework#Due_M3D1| Homework due]] <br> | ||
|-- | |-- | ||
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| 2 | | 2 | ||
| R/F Apr 16/17 | | R/F Apr 16/17 | ||
− | | BE Comm Lab<br> | + | | BE Comm Lab<br>[https://mit.zoom.us/j/714801029 Zoom access]<br>[https://mit.zoom.us/rec/share/vO4sCK3R13lLQLPz92_FfqdmMMfGT6a81SFM8_YEzxlVVanXWhZYdlr9o1t1XnjW M3D2 video] |
− | | [[20.109(S20):Harvest candidate clones (Day2) |Harvest candidate clones and prepare for sequencing]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | | [[20.109(S20):Harvest candidate clones (Day2) |Harvest candidate clones and prepare for sequencing]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access], [https://mit.zoom.us/rec/share/xeNpF6qhy2xJBavK6WXScZ8vN5_feaa8hClN-fALyxkSLXnS-p3m-4ygYoUueYQP T/R prelab video], [[Media:Sp20 M3D2 nll.pdf|T/R prelab slides]]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/5-lMFazw7k9OE7Pv1WCHWo0sNYW6eaa8hyZIrvUNnRq4XRYFKqIql9nUCBDsmA_Z W/F prelab video], [[Media:Sp20 M3D2 bcm unmarked.pdf | WF prelab slides]] |
| [[20.109(S20):Homework#Due_M3D2| Homework due]] <br> | | [[20.109(S20):Homework#Due_M3D2| Homework due]] <br> | ||
|-- | |-- | ||
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| 3 | | 3 | ||
| R/F Apr 23/24 | | R/F Apr 23/24 | ||
− | | [http://be.mit.edu/directory/leslie-mcclain LM]<br> | + | | [http://be.mit.edu/directory/leslie-mcclain LM]<br>[https://mit.zoom.us/s/613303093 Zoom access]<br>[[Media:Sp20_Mod3_lecture 2.pdf|M3D3 slides]]<br>[https://mit.zoom.us/rec/share/vPEkMOnSyXNLWKeRzhDuB6MfQ6viX6a80yFM-PNZzBx2cnJ6YG_9C7zvCKKs4QjS?startTime=1587654517000 M3D3 video] |
− | | [[20.109(S20):Identify clones to characterize (Day3) |Identify clones to characterize]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | | [[20.109(S20):Identify clones to characterize (Day3) |Identify clones to characterize]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access], [https://mit.zoom.us/rec/share/9dcuKu-v2m9JRp3f2EzuUYkuJ638eaa81HIZ-_IIzh2hRtewr_XwzEtoM7qdju7B?startTime=1587661822000 T/R prelab video], [[Media:Sp20 M3D3 nll.pdf|T/R prelab slides]] <br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/upBYIPLp3EdOYaPStlmcRP9_BsP5X6a8hHAcrvYPzZq5X1fC51L1AqfP8gZyNX0?startTime=1587748234000 W/F prelab video], [[Media:Sp20 M3D3 bcm.pdf | WF Prelab slides]] <br> |
| <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D3| Homework due]] <br> | | <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D3| Homework due]] <br> | ||
|-- | |-- | ||
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| | | | ||
| T/W Apr 28/29 | | T/W Apr 28/29 | ||
− | | [http://be.mit.edu/directory/leslie-mcclain LM] | + | | [http://be.mit.edu/directory/leslie-mcclain LM]<br>[https://mit.zoom.us/s/613303093 Zoom access]<br>[[Media:Sp20_M3L3_slides_handout.pdf|Apr28 slides/handout]]<br>[https://mit.zoom.us/rec/share/3dRJJbHQ1mpORq_wuGeCY4czEo7IX6a8gyRI-KVfyRtzNY-7ruAaJPIHg-M7mN8F?startTime=1588086584000 Apr28 video] |
− | | Research proposal pitches, will be | + | | Research proposal pitches, will be delivered by each team during laboratory time <br> [https://mit.zoom.us/j/137247921 T/R Zoom access]<br>[https://mit.zoom.us/j/735216560 W/F Zoom access] |
| | | | ||
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| 4 | | 4 | ||
| R/F Apr/May 30/1 | | R/F Apr/May 30/1 | ||
− | | [http://be.mit.edu/directory/leslie-mcclain LM]<br> | + | | [http://be.mit.edu/directory/leslie-mcclain LM]<br>[https://mit.zoom.us/s/613303093 Zoom access]<br>[[Media:Sp20_M3L4.pdf|M3D4 slides]]<br>[https://mit.zoom.us/rec/share/zP5QF7Pu6XJOXYnQ8mPgaJAYQIW6T6a8hyQYq6cEzEfHjTEa-Gql4TCi8MxquSrH?startTime=1588259394000 M3D4 video] |
− | | [[20.109(S20):Characterize clone by titration using flow cytometry (Day4)|Characterize clone by titration using flow cytometry ]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | | [[20.109(S20):Characterize clone by titration using flow cytometry (Day4)|Characterize clone by titration using flow cytometry ]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access], [ T/R prelab video], [[Media:Sp20 M3D4 nll.pdf|T/R prelab slides]] <br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/zMdNAZXR8HxLR9Kd826cZoF6HIjleaa8gSYXrqFfyxxkaqhSmmOHldJDtxTRw4MN WF prelab video], [[Media:Sp20 M3D4 bcm unmarked.pdf | WF prelab slides]] |
| [[20.109(S20):Homework#Due_M3D4| Homework due]] | | [[20.109(S20):Homework#Due_M3D4| Homework due]] | ||
|-- | |-- | ||
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| 5 | | 5 | ||
| T/W May 5/6 | | T/W May 5/6 | ||
− | | Guest, SC | + | | Guest, SC<br>[https://mit.zoom.us/s/613303093 Zoom access]<br>[[Media:200505_SCC_guestLecture_notPublic.pdf|M3D5 Slides]]<br>[https://mit.zoom.us/rec/share/4-5FK4Gh0XxITJ314xCYQekQEqrieaa80XdI-vJeyF_vwbqD09tY0wQ323CJNEo?startTime=1588691427000 M3D5 video] |
− | | [[20.109(S20):Analyze titration curves (Day5) |Analyze titration curves ]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access] | + | | [[20.109(S20):Analyze titration curves (Day5) |Analyze titration curves ]]<br>[https://mit.zoom.us/j/137247921 T/R Zoom access], [ T/R prelab video], [[Media:Sp20 M3D5 nll.pdf|T/R prelab slides]]<br> [https://mit.zoom.us/j/735216560 W/F Zoom access], [https://mit.zoom.us/rec/share/vOtbdbje63pOQoWKxRjzevQZAd7FT6a82nMYq_tfzx6w7ESzcewfbUI6OAqq7ZSF W/F prelab video], [[Media:Sp20 M3D5 bcm unmarked.pdf | WF prelab slides]] |
− | | [[20.109(S20):Homework#Due_M3D5| Homework due]] | + | | <font color= #01A9DB>'''Laboratory quiz'''</font color> <br>[[20.109(S20):Homework#Due_M3D5| Homework due]] |
|-- | |-- | ||
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| R/F May 7/8 | | R/F May 7/8 | ||
| | | | ||
− | | [[20.109(S20):Research proposal presentation | <font color=#B40404>'''Research proposal presentations'''</font color>]], will be | + | | [[20.109(S20):Research proposal presentation | <font color=#B40404>'''Research proposal presentations'''</font color>]], will be delivered by each team during laboratory time<br> [https://mit.zoom.us/j/137247921 T/R Zoom access]<br>[https://mit.zoom.us/j/735216560 W/F Zoom access] |
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Latest revision as of 21:02, 6 May 2020
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (56-154)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)