Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"
From Course Wiki
(→T/R) |
(→Fermentation product and gene targeted:) |
||
(27 intermediate revisions by 13 users not shown) | |||
Line 11: | Line 11: | ||
|yellow | |yellow | ||
|L | |L | ||
− | | | + | |pflB |
− | | | + | |TGTCGAAGTACGCAGTAAAT |
|- | |- | ||
|green | |green | ||
− | | | + | |L |
− | | | + | |pflB |
− | | | + | |ATAAAAAATCCACTTAAGAAGGTA |
|- | |- | ||
|blue | |blue | ||
− | | | + | |E |
− | | | + | |pta |
− | | | + | |ttcacgacaacgttcaataatcat |
|- | |- | ||
|pink | |pink | ||
− | | | + | |L |
− | | | + | |pflB |
− | | | + | |AAATAAAAAATCCACTTAAGAAGGT |
|- | |- | ||
|purple | |purple | ||
− | | | + | |L |
− | | | + | |pflB |
− | | | + | |AAATCCACTTAAGAAGGTAGGTGT |
+ | |white | ||
+ | |L | ||
+ | |pflB | ||
+ | |AAATAAAAAATCCACTTAAGAAGGT | ||
+ | |- | ||
|} | |} | ||
Line 44: | Line 49: | ||
|- | |- | ||
|red | |red | ||
− | | | + | |E |
− | | | + | |pta-ack |
− | | | + | |GCC ACG TAT CAA TTA TAG GTA C |
|- | |- | ||
|green | |green | ||
− | | | + | |E |
− | | | + | |ldhA |
− | | | + | |GTAGCTTAAATGTGATTCAACATC |
|- | |- | ||
|blue | |blue | ||
− | | | + | |E |
− | | | + | |fdrA |
− | | | + | |CAAGATCGGCTTGAAAGGTTTGCAC |
|- | |- | ||
|purple | |purple | ||
− | | | + | |E |
− | | | + | |ldhA |
− | | | + | |TCGTACTGTTTTGTGCTATAAA |
|} | |} |
Latest revision as of 19:08, 17 October 2017
Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | ||||
yellow | L | pflB | TGTCGAAGTACGCAGTAAAT | ||||
green | L | pflB | ATAAAAAATCCACTTAAGAAGGTA | ||||
blue | E | pta | ttcacgacaacgttcaataatcat | ||||
pink | L | pflB | AAATAAAAAATCCACTTAAGAAGGT | ||||
purple | L | pflB | AAATCCACTTAAGAAGGTAGGTGT | white | L | pflB | AAATAAAAAATCCACTTAAGAAGGT |
W/F
Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
red | E | pta-ack | GCC ACG TAT CAA TTA TAG GTA C |
green | E | ldhA | GTAGCTTAAATGTGATTCAACATC |
blue | E | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC |
purple | E | ldhA | TCGTACTGTTTTGTGCTATAAA |