Difference between revisions of "Talk:20.109(F17):Module 1"
From Course Wiki
(→CometChip Data) |
(→CometChip Data) |
||
(2 intermediate revisions by one user not shown) | |||
Line 4: | Line 4: | ||
=CometChip Data= | =CometChip Data= | ||
− | The ' | + | The '''Analyzed Data''' below refer to Excel spreadsheets you will upload, in which |
*Rows A, B, C were triplicates of wild type MEF cells | *Rows A, B, C were triplicates of wild type MEF cells | ||
*Rows D, E, F were triplicates of mutant MEF cells | *Rows D, E, F were triplicates of mutant MEF cells | ||
Line 12: | Line 12: | ||
*The 'comet number' tab indicates how many comets were analyzed in each image. | *The 'comet number' tab indicates how many comets were analyzed in each image. | ||
*The other tabs represent various options to quantify DNA damage. | *The other tabs represent various options to quantify DNA damage. | ||
+ | |||
+ | |||
+ | The '''Images''' column contains fluorescence images of the CometChips taken at 4x magnification. One or two images were taken per macrowell. | ||
+ | |||
{| border=1px | {| border=1px |
Latest revision as of 17:38, 2 October 2017
CometChip Data
The Analyzed Data below refer to Excel spreadsheets you will upload, in which
- Rows A, B, C were triplicates of wild type MEF cells
- Rows D, E, F were triplicates of mutant MEF cells
- Columns 1-6 correspond to the 6 drug doses the cells were treated with:
- H2O2: 0, 5, 10, 15, 20, 25 μM
- MMS: 0, 0.1, 0.2, 0.5, 6, and 8 mM.
- The 'comet number' tab indicates how many comets were analyzed in each image.
- The other tabs represent various options to quantify DNA damage.
The Images column contains fluorescence images of the CometChips taken at 4x magnification. One or two images were taken per macrowell.
Team | Analyzed Data | Images |
T/R red | buffer_data enzyme_data | buffer_part1 buffer_part2 enzyme_part1 enzyme_part2 |
T/R orange | buffer_data enzyme_data | all_images |
T/R yellow | buffer_data enzyme_data | buffer_part1 buffer_part2 enzyme_part1 enzyme_part2 |
T/R green | buffer_data enzyme_data | all_images |
T/R blue | buffer_data enzyme_data | buffer_part1 buffer_part2 enzyme_part1 enzyme_part2 |
T/R pink | buffer_data enzyme_data | all_images |
T/R purple | buffer_data enzyme_data | all_images |
T/R white | buffer_data enzyme_data | buffer_part1 buffer_part2 enzyme_part1 enzyme_part2 |
W/F red | buffer_data enzyme_data | all_images |
W/F orange | buffer_data enzyme_data | all_images |
W/F green | buffer_data enzyme_data | buffer_part1 buffer_part2 enzyme_part1 enzyme_part2 |
W/F blue | buffer_data enzyme_data | buffer_part1 buffer_part2 enzyme_part1 enzyme_part2 |