Difference between revisions of "QRT-PCR"
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Revision as of 01:08, 25 February 2011
Project Proposal
GOAL: be able to detect a trace amount of known DNA
Tasks:
- cyclic heating
- heated lid
- accurate and uniform heating
- sufficiently rapid cooling
- accurate temperature readout
- fluorescence detection
- optics design and setup
- electronics: amplification of signal, noise reduction
- coding
- heating control
- mathifying the input data (fluoresence, etc) to produce useful information (# amplicons)
- GUI
Questions:
- exactly how accurate do we need to be?
- how fast is fast enough for cooling?
