Difference between revisions of "ThorLabs OTKB optical trapping kit"
From Course Wiki
(→Position calibration) |
(→Comparison of OTKB and 20.309 optical trap operating characteristics) |
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===Position calibration=== | ===Position calibration=== | ||
− | The position calibrations | + | The position calibrations determine the sensitivity of the quadrant photodiode detector to changes in position of a bead. Accurate quantitative results depend on precise position calibration. |
====Methodology==== | ====Methodology==== | ||
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===Trap stiffness=== | ===Trap stiffness=== | ||
+ | The force calibrations determine the stiffness of the trap. The stiffness of the trap characterizes the forces exerted by it. Three different methods were used in order to determine the stiffness of each trap: Equipartition, noise PSD roll-off, and Stokes drag. | ||
+ | |||
+ | ====Methodology==== | ||
+ | |||
+ | ====Results==== | ||
+ | |||
+ | =====Equipartition===== | ||
+ | |||
+ | {| border="1" textalign="center" | ||
+ | |- | ||
+ | ! | ||
+ | !ThorLabs OTKB | ||
+ | !20.309 | ||
+ | |- | ||
+ | !X and Y axes | ||
+ | |TBD | ||
+ | |[[Image:Equipartition.gif|300px|X and Y axis Force Calibration]] |
Revision as of 18:31, 6 July 2010
Contents
OTKB construction
Modifications to ThorLabs suggested layout
Alignment procedure
Comparison of OTKB and 20.309 optical trap operating characteristics
Position calibration
The position calibrations determine the sensitivity of the quadrant photodiode detector to changes in position of a bead. Accurate quantitative results depend on precise position calibration.
Methodology
Results
ThorLabs OTKB | 20.309 | |
---|---|---|
X position | TBD | |
Y position | TBD |
Trap stiffness
The force calibrations determine the stiffness of the trap. The stiffness of the trap characterizes the forces exerted by it. Three different methods were used in order to determine the stiffness of each trap: Equipartition, noise PSD roll-off, and Stokes drag.
Methodology
Results
Equipartition
ThorLabs OTKB | 20.309 | |
---|---|---|
X and Y axes | TBD |