Difference between revisions of "20.109(S13):Context-setting and primer design (Day1)"
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==Introduction== | ==Introduction== | ||
| − | overall motivation | + | *overall motivation |
| − | + | Identifying pathogens in humans can suggest appropriate course of treatment. Identifying pathogens in other species can be both intellectually interesting and have applications for human health | |
| − | + | Molecular identification of pathogens as powerful and perhaps less subjective than the identification according to morphology and phenotype | |
| − | + | Requires a highly conserved gene. Something essential and little tolerance of mutation. Hence rRNA. | |
| − | primer design basic background here or directly in protocols? | + | [Clarridge review] |
| + | |||
| + | *background about small subunit rRNA | ||
| + | |||
| + | |||
| + | *bacteria-specific background | ||
| + | |||
| + | *microsporidia-specific background | ||
| + | |||
| + | Over 1000 species, with 14 infecting humans | ||
| + | |||
| + | Not a time of full genome sequence information | ||
| + | |||
| + | [Broad initiative] | ||
| + | |||
| + | Cites for bird infections and zoonotic potential | ||
| + | |||
| + | Parallels to avian flu | ||
| + | |||
| + | *primer design basic background here or directly in protocols? | ||
==Protocols== | ==Protocols== | ||
Revision as of 19:38, 13 December 2012
Contents
Introduction
- overall motivation
Identifying pathogens in humans can suggest appropriate course of treatment. Identifying pathogens in other species can be both intellectually interesting and have applications for human health
Molecular identification of pathogens as powerful and perhaps less subjective than the identification according to morphology and phenotype
Requires a highly conserved gene. Something essential and little tolerance of mutation. Hence rRNA.
[Clarridge review]
- background about small subunit rRNA
- bacteria-specific background
- microsporidia-specific background
Over 1000 species, with 14 infecting humans
Not a time of full genome sequence information
[Broad initiative]
Cites for bird infections and zoonotic potential
Parallels to avian flu
- primer design basic background here or directly in protocols?
Protocols
Part 1: Lab practical
You and your partner may work together on the lab practical. (Note: this will not be the case for future quizzes.) You are of course welcome to give different answers should you disagree.
