Difference between revisions of "20.109(F08): Fall 2008 schedule"
From Course Wiki
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| Tue Sept 9 | | Tue Sept 9 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08):Start-up genome engineering | DNA engineering using PCR]] |
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|-- | |-- | ||
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| Thur Sept 11 | | Thur Sept 11 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): Agarose gel electrophoresis | Clean and cut DNA]] |
| | | | ||
|-- | |-- | ||
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| Tue Sept 16 | | Tue Sept 16 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): DNA ligation and bacterial transformation| Agarose gel electrophoresis]] |
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|-- | |-- | ||
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| Thur Sept 18 | | Thur Sept 18 | ||
| [[http://web.mit.edu/be/people/engelward.htm BE] | | [[http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): Examine candidate clones| DNA ligation and bacterial transformation]] |
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|-- | |-- | ||
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| Tue Sept 23 | | Tue Sept 23 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): M13.1| Examine candidate clones]] |
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|-- | |-- | ||
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| Thur Sept 25 | | Thur Sept 25 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): Western analysis| Restriction map and tissue culture]] |
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|-- | |-- | ||
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| Tue Sept 30 | | Tue Sept 30 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): Probe western| Lipofection]] |
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|-- | |-- | ||
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| Thur Oct 2 | | Thur Oct 2 | ||
| [http://web.mit.edu/be/people/engelward.htm BE] | | [http://web.mit.edu/be/people/engelward.htm BE] | ||
− | | | + | | [http://openwetware.org/wiki/User:Michelle_R._Sukup MS] |
− | | [[20.109( | + | | [[20.109(F08): Module 1 oral presentations| FACS analysis]] |
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|-- | |-- | ||
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| Tues Oct 7 | | Tues Oct 7 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): siRNA design| Protein engineering with PCR]] |
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|-- | |-- | ||
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| Thur Oct 9 | | Thur Oct 9 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): Transfection| Yeast transformation]] |
| | | | ||
|-- | |-- | ||
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| Tues Oct 14 | | Tues Oct 14 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): Luciferase assays and RNA prep| Colony PCR, Journal article discussion]] |
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|-- | |-- | ||
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| Thur Oct 16 | | Thur Oct 16 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): Journal article discussion| SDS-PAGE, screen for phenotypes]] |
| Guest lecture: [[User:Nlerner| NL]] from the writing program. | | Guest lecture: [[User:Nlerner| NL]] from the writing program. | ||
|- | |- | ||
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| Tues Oct 21 | | Tues Oct 21 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): cDNA synthesis and microarray| Probe Western, isolate RNA]] |
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|-- | |-- | ||
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| Thurs Oct 23 | | Thurs Oct 23 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): Microarray data analysis| Journal Club I]] |
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|-- | |-- | ||
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| Tues Oct 28 | | Tues Oct 28 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | | + | | [[20.109(F08): Mod 2 Day 7| cDNA synthesis and microarray]] |
| | | | ||
|-- | |-- | ||
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| Thurs Oct 30 | | Thurs Oct 30 | ||
| [[Natalie Kuldell | NK]] | | [[Natalie Kuldell | NK]] | ||
− | | | + | | [http://openwetware.org/wiki/User:Brian_Belmont BB] |
− | | [[20.109( | + | | [[20.109(F08): Module 2 oral presentations| Microarray data analysis]] |
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| Tues Nov 4 | | Tues Nov 4 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | | [http://openwetware.org/wiki/Luis_Alvarez LA] |
− | | [[20.109(F08): Growth of phage materials| | + | | [[20.109(F08): Growth of phage materials| Phage growth]] |
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|-- | |-- | ||
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| Tue Nov 6 | | Tue Nov 6 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | | [http://openwetware.org/wiki/Luis_Alvarez LA] |
| [[20.109(F08): Phage nanowires| Phage nanowires]] | | [[20.109(F08): Phage nanowires| Phage nanowires]] | ||
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| Thur Nov 13 | | Thur Nov 13 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | | [http://openwetware.org/wiki/Luis_Alvarez LA] |
− | | [[20.109(F08): Transmission electron microscopy| | + | | [[20.109(F08): Transmission electron microscopy| Journal Club II]] |
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|-- | |-- | ||
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| Tue Nov 18 | | Tue Nov 18 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | | [http://openwetware.org/wiki/Luis_Alvarez LA] |
| [[20.109(F08): Phage by design| Phage by design]] | | [[20.109(F08): Phage by design| Phage by design]] | ||
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| Thur Nov 20 | | Thur Nov 20 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | | [http://openwetware.org/wiki/Luis_Alvarez LA] |
| [[20.109(F08): Phage by design, pt2| Phage by design, pt 2]] | | [[20.109(F08): Phage by design, pt2| Phage by design, pt 2]] | ||
| | | | ||
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| Tue Dec 2 | | Tue Dec 2 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | | [http://openwetware.org/wiki/Luis_Alvarez LA] |
| [[20.109(F08): ECD assembly| ECD assembly]] | | [[20.109(F08): ECD assembly| ECD assembly]] | ||
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| Thur Dec 4 | | Thur Dec 4 | ||
| [[User:Abelcher| AB]] | | [[User:Abelcher| AB]] | ||
− | | | + | |[http://openwetware.org/wiki/Luis_Alvarez LA] |
| [[20.109(F08): Module 3 oral presentations| Oral presentations]] | | [[20.109(F08): Module 3 oral presentations| Oral presentations]] | ||
| | | |
Revision as of 00:07, 24 July 2008
MODULE | DAY | DATE | LECTURER | TA | LAB | note |
Thur Sept 4 | NK | Orientation | ||||
1 | 1 | Tue Sept 9 | BE | MS | DNA engineering using PCR | |
1 | 2 | Thur Sept 11 | BE | MS | Clean and cut DNA | |
1 | 3 | Tue Sept 16 | BE | MS | Agarose gel electrophoresis | |
1 | 4 | Thur Sept 18 | [BE | MS | DNA ligation and bacterial transformation | |
1 | 5 | Tue Sept 23 | BE | MS | Examine candidate clones | |
1 | 6 | Thur Sept 25 | BE | MS | Restriction map and tissue culture | |
1 | 7 | Tue Sept 30 | BE | MS | Lipofection | |
1 | 8 | Thur Oct 2 | BE | MS | FACS analysis | |
2 | 1 | Tues Oct 7 | NK | BB | Protein engineering with PCR | |
2 | 2 | Thur Oct 9 | NK | BB | Yeast transformation | |
2 | 3 | Tues Oct 14 | NK | BB | Colony PCR, Journal article discussion | |
2 | 4 | Thur Oct 16 | NK | BB | SDS-PAGE, screen for phenotypes | Guest lecture: NL from the writing program. |
2 | 5 | Tues Oct 21 | NK | BB | Probe Western, isolate RNA | |
2 | 6 | Thurs Oct 23 | NK | BB | Journal Club I | |
2 | 7 | Tues Oct 28 | NK | BB | cDNA synthesis and microarray | |
2 | 8 | Thurs Oct 30 | NK | BB | Microarray data analysis | |
3 | 1 | Tues Nov 4 | AB | LA | Phage growth | |
3 | 2 | Tue Nov 6 | AB | LA | Phage nanowires | |
Veteran's Day Break Nov 10,11 | No lab Tuesday and Wednesday | |||||
---|---|---|---|---|---|---|
3 | 3 | Thur Nov 13 | AB | LA | Journal Club II | |
3 | 4 | Tue Nov 18 | AB | LA | Phage by design | |
3 | 5 | Thur Nov 20 | AB | LA | Phage by design, pt 2 | |
Thanksgiving Break Nov | No lab or lecture all week | |||||
3 | 6 | Tue Dec 2 | AB | LA | ECD assembly | |
3 | 7 | Thur Dec 4 | AB | LA | Oral presentations | |
3 | 8 | Tue Dec 9 | end of term celebration... | Lecture, no lab, for class evaluations then lunch from noon-1:30 PM, |