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Line 49: |
Line 49: |
| ===Green Team=== | | ===Green Team=== |
| *Team members: Arjun Naskar, Patrick Alayon | | *Team members: Arjun Naskar, Patrick Alayon |
− | *Description of M13.1 phage: | + | *Description of M13.1 phage: p3F17Ag |
| *Description of MDS strain: MDS60 | | *Description of MDS strain: MDS60 |
| *Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen. | | *Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen. |
Revision as of 21:29, 21 December 2007
20.109(F07): Laboratory Fundamentals of Biological Engineering
All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
10-6
|
|
|
|
|
10-8
|
|
|
|
|
10-10
|
|
|
|
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
titer (PFU/ml)
|
|
|
|
|
T/R lab
Green Team
- Team members: Arjun Naskar, Patrick Alayon
- Description of M13.1 phage: p3F17Ag
- Description of MDS strain: MDS60
- Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen.
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
10-6
|
~1000
|
ND
|
0(uneven)
|
0
|
10-8
|
0 (uneven)
|
ND
|
0
|
0
|
10-10
|
0
|
ND
|
0
|
0
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
titer (PFU/ml)
|
|
|
|
|
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here: no phage control has plaques
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
10-6
|
|
|
|
|
10-8
|
|
|
|
|
10-10
|
|
|
|
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
titer (PFU/ml)
|
|
|
|
|
Red Team
- Team members:Rosa Alvarez, Vivian Hernandez
- Description of M13.1 phage:p9-YFP
- Description of MDS strain:MDS42
- Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
10-6
|
fully lysed
|
ND
|
0
|
0
|
10-8
|
~1000
|
ND
|
0
|
0
|
10-10
|
~150
|
ND
|
0
|
0
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
titer (PFU/ml)
|
|
|
|
|
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Blue Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:M13K07 control not grown in parallel
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
10-6
|
~1000
|
ND
|
~1000
|
~1500
|
10-8
|
16
|
ND
|
10
|
42
|
10-10
|
|
|
|
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
titer (PFU/ml)
|
|
|
|
|
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
W/F lab
Green Team
- Team members: Hao Ding and Neha Batra
- Description of M13.1 phage: p8::BAP
- Description of MDS strain: MDS12
- Any notes about titers presented here: Did not get culture for MDS12xM13.1, ctrl showed no plaques (no contamination)
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS 12
|
phage = M13.1, strain = MG1655
|
10-6
|
>800
|
>800
|
14
|
10-8
|
74
|
63
|
1 (maybe)
|
10-10
|
1 (maybe)
|
0
|
0
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
titer (PFU/ml)
|
7.6e11 PFU/ml
|
6.3e11 PFU/ml
|
1.4e9 PFU/ml
|
Purple Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Red Team
- Team members: Scott Pollom and Spenser Skates
- Description of M13.1 phage: dual p8
- Description of MDS strain: MDS42
- Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. We used the 10^-8 dilution data to calculate the three PFU/ml values.
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
10-6
|
too many
|
ND
|
800
|
too many
|
10-8
|
174
|
ND
|
113
|
225
|
10-10
|
350
|
ND
|
1
|
231
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS
|
titer (PFU/ml)
|
1.74e12 PFU/ml
|
ND
|
1.13e12 PFU/ml
|
2.25e12 PFU/ml
|
Pink Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here:
Plaque numbers
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS 43
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS 43
|
10-6
|
2400
|
1632
|
17
|
27
|
10-8
|
78
|
43
|
68
|
25
|
10-10
|
2
|
2
|
31
|
49
|
Titer
|
phage = M13K07, strain = MG1655
|
phage = M13K07, strain = MDS43
|
phage = M13.1, strain = MG1655
|
phage = M13.1, strain = MDS43
|
titer (PFU/ml)
|
2.4E11 PFU/ml
|
1.63E11 PFU/ml
|
1.7E9 PFU/ml
|
2.7E9 PFU/ml
|
Blue Team
- Team members: Matthew Loper and Kat Pak
- Description of M13.1 phage:
- Description of MDS strain: MDS43
- Any notes about titers presented here: negative control showed 62 plaques so there was probably contaimination
Yellow Team
- Team members:
- Description of M13.1 phage:
- Description of MDS strain:
- Any notes about titers presented here: